Taxol (paclitaxel) production using free and immobilized cells of Taxus cuspidata.
Taxol (paclitaxel) production using free and immobilized cells of Taxus cuspidata.
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使用东北红豆杉的游离和固定细胞生产紫杉醇（紫杉醇）。
DOI：
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发表时间：
1997
影响因子：
3.8
通讯作者：
S. Furusaki
中科院分区：
文献类型：
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作者：
M. Seki;C. Ohzora;M. Takeda;S. Furusaki
在灌流培养生物反应器中研究了东北红豆杉游离细胞和固定化细胞生产紫杉醇的特性。虽然细胞生长受到较高稀释率的抑制，但与分批操作相比，灌注操作提高了紫杉醇的比生产速率。使用尼龙网细胞分离器的自由悬浮细胞的灌注培养显示出类似的生产概况，使用固定化细胞获得的。连续紫杉醇生产成功地获得了约0.3毫克/克DCW（干细胞重量）每天的特定生产率长达40天。(c)John Wiley & Sons，Inc.
The production characteristics for Taxol (paclitaxel) using free and immobilized cells of Taxus cuspidata were investigated in a perfusion culture bioreactor. Although the cell growth was inhibited by higher dilution rates, the specific production rate of Taxol was increased by perfusion compared with that using batch operation. Perfusion cultures using a nylon-mesh cell separator for free suspension cells showed similar production profiles to those obtained using immobilized cells. Continuous Taxol production was successfully obtained at an approximate specific production rate of 0.3 mg/g DCW (dry cell weight) per day for up to 40 days. (c) 1997 John Wiley & Sons, Inc.
影响因子：
5.1
作者：
Witherup,KM;Look,SA;Stasko,MW;Ghiorzi,TJ;Muschik,GM;Cragg,GM
通讯作者：
Cragg,GM