Multi-parameter fluorescence-activated cell sorting and analysis of stem and progenitor cells in myeloid malignancies.
Multi-parameter fluorescence-activated cell sorting and analysis of stem and progenitor cells in myeloid malignancies.
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DOI：
10.1016/j.beha.2010.06.006
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发表时间：
2010-09
影响因子：
2.1
通讯作者：
Steidl, Ulrich
中科院分区：
文献类型：
--
作者：
Will, Britta;Steidl, Ulrich
关键词：
由于发现罕见的白血病起始细胞（或白血病干细胞，LSC）起源于并繁殖高度分化的白血病母细胞的分级细胞组织，因此精确定义的干细胞和祖细胞的分析变得越来越重要。用于造血祖细胞亚分级分离的多参数高速荧光激活细胞分选（FACS）的出现允许对LSC的起源细胞的生物学和LSC作为可能逃避化疗并因此导致疾病复发的潜在（或必要）治疗靶点的生物学进行研究。本文介绍了目前最先进的造血干细胞和祖细胞的分级分离方法，特别关注髓系恶性肿瘤。由于人类正常和恶性造血的许多方面经常在动物研究中建模，我们还提供了造血干细胞和祖细胞纯化方法的概述，这些方法通常用于在小鼠疾病模型中的研究。
Owing to the discovery that rare leukemia-initiating cells (or leukemia stem cells, LSC) give origin to and propagate a hierarchical cellular organization of variably differentiated leukemic blasts, the analysis of precisely defined stem and progenitor cells has increasingly gained importance. Emergence of multi-parameter high-speed fluorescence-activated cell sorting (FACS) for the subfractionation of hematopoietic progenitor cells has allowed research on the biology of the cell-of-origin for LSCs and of LSCs as potential (or essential) therapeutic targets that may escape chemotherapy and consequently contribute to disease relapse. This review introduces the current state-of-the-art methods for the fractionation of hematopoietic stem and progenitor cells, with particular focus on myeloid malignancies. As many aspects of human normal and malignant hematopoiesis are frequently modeled in animal studies, we also provide an overview of hematopoietic stem and progenitor cell purification methods that are commonly utilized for research in murine models of disease.
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