Mouse hematopoietic stem cell identification and analysis.
Mouse hematopoietic stem cell identification and analysis.
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DOI：
10.1002/cyto.a.20674
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发表时间：
2009-01
期刊：
影响因子：
3.7
通讯作者：
Goodell, Margaret A.
中科院分区：
文献类型：
--
作者：
Challen, Grant A.;Boles, Nathan;Lin, Karen Kuan-Yin;Goodell, Margaret A.
造血干细胞(HSCs)仍然是迄今为止最具特征的成体干细胞群体，无论是在纯化标志方面还是在评估功能潜力的检测方面都是如此。然而，尽管经过了40多年的研究，在小鼠体内使用造血干细胞仍然很困难，因为这些细胞在骨髓中相对丰富(或缺乏)。HSCs在骨髓中的比例约为有核细胞总数的0.01%，根据小鼠的年龄、性别和品系以及所采用的纯化方案，每只小鼠可分离出约5000个HSCs。这禁止了对需要大量起始材料的高速干细胞中工艺的研究。增加挑战的是不断有新的HSC纯化标志物的报道，这使得该领域的外行很难知道哪种纯化策略产生的长期、多系HSC比例最高。本报告将综述不同的造血干和造血祖细胞纯化策略，并比较不同仪器对HSC分选和分析的流式细胞术图谱。我们还将讨论外周血细胞类型的快速流式细胞术分析方法，以及在通过BrdU、Ki-67和派罗宁Y染色分析细胞周期状态时使用稀有细胞群(如HSCs)的新策略。这篇综述的目的是对小鼠造血干细胞生物学的一些最新实验和技术进展提供洞察。
Hematopoietic stem cells (HSCs) remain by far the most well-characterized adult stem cell population both in terms of markers for purification and assays to assess functional potential. However, despite over 40 years of research, working with HSCs in the mouse remains difficult because of the relative abundance (or lack thereof) of these cells in the bone marrow. The frequency of HSCs in bone marrow is about 0.01% of total nucleated cells and approximately 5000 can be isolated from an individual mouse depending on the age, sex and strain of mice as well as purification scheme utilized. This prohibits the study of processes in HSCs which require large amounts of starting material. Adding to the challenge is the continual reporting of new markers for HSC purification, which makes it difficult for the uninitiated in the field to know which purification strategies yield the highest proportion of long-term, multi-lineage HSCs. This report will review different hematopoietic stem and progenitor purification strategies and compare flow cytometry profiles for HSC sorting and analysis on different instruments. We will also discuss methods for rapid flow cytometric analysis of peripheral blood cell types, and novel strategies for working with rare cell populations such as HSCs in the analysis of cell cycle status by BrdU, Ki-67 and Pyronin Y staining. The purpose of this review is to provide insight into some of the recent experimental and technical advances in mouse hematopoietic stem cell biology.
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影响因子：
64.5
作者：
Kiel, MJ;Yilmaz, ÖH;Morrison, SJ
通讯作者：
Morrison, SJ
影响因子：
2.6
作者：
Novelli, EM;Ramirez, M;Civin, CI
通讯作者：
Civin, CI
影响因子：
3.7
作者：
Sharma, Siddharth;Cabana, Raquel;Krishan, Awtar
通讯作者：
Krishan, Awtar
影响因子：
20.3
作者：
Camargo, FD;Chambers, SM;Goodell, MA
通讯作者：
Goodell, MA
DOI：
10.1084/jem.20050967
发表时间：
2005-12-05
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Passegué E;Wagers AJ;Giuriato S;Anderson WC;Weissman IL
通讯作者：
Weissman IL