Regulation of migratory activity of human keratinocytes by topography of multiscale collagen-containing nanofibrous matrices.
Regulation of migratory activity of human keratinocytes by topography of multiscale collagen-containing nanofibrous matrices.
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DOI：
10.1016/j.biomaterials.2013.11.013
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发表时间：
2014-03
期刊：
影响因子：
14
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Fu X;Xu M;Liu J;Qi Y;Li S;Wang H
纳米纤维基质在皮肤伤口修复中具有很大的前景，部分原因是它们能够重新获得天然细胞外基质（ECM）的基本属性。关于纳米纤维基质对角质形成细胞影响的研究有限，本研究旨在通过在聚己内酯(PCL)/胶原纳米纤维基质（纤维直径为331±112 nm的粗糙表面）或涂有薄层胶原凝胶的基质上形成次级超细纤维网络（超细纤维直径为55±26 nm的光滑表面）上培养角质形成细胞，了解纳米纤维基质的地形特征如何调节角质形成细胞的运动。结果发现，单独使用PCL/胶原纳米纤维基质对细胞迁移没有刺激作用，而胶原凝胶包被可显著提高细胞的运动性。进一步研究表明，胶原凝胶包被的超细纤维网络显著激活整合素β1、Rac1和Cdc42，促进层粘连蛋白332（以前称为层粘连蛋白5）的沉积，促进活性基质金属蛋白酶（MMPs）（即MMP-2和9）的表达。整合素β1活性的中和消除了凝胶涂层诱导的角质细胞迁移。这些发现为纳米纤维基质的地形特征在调节角质形成细胞表型改变中的作用提供了重要证据，并表明含有胶原的纳米纤维基质在皮肤再生特别是再上皮化中的可能用途。
Nanofibrous matrices hold great promise in skin wound repair partially due to their capability of recapturing the essential attributes of native extracellular matrix (ECM). With regard to limited studies on the effect of nanofibrous matrices on keratinocytes, the present study was aimed to understand how the topographical feature of nanofibrous matrices regulates keratinocyte motility by culturing keratinocytes on polycaprolactone (PCL)/collagen nanofibrous matrices (rough surface with fiber diameters of 331±112 nm) or the matrices coated with a thin layer of collagen gel to form a secondary ultrafine fibrous network (smooth surface with ultrafine fiber diameters of 55±26 nm). It was found that the PCL/collagen nanofibrous matrices alone did not stimulate cell migration, while collagen gel coating could significantly increase cell motility. Further studies demonstrated that the ultrafine fibrous network of collagen gel coating significantly activated integrin β1, Rac1 and Cdc42, facilitated the deposition of laminin-332 (formerly called laminin-5), and promoted the expression of active matrix metalloproteinases (MMPs) (i.e., MMP-2 and 9). Neutralization of integrin β1 activity abrogated the gel coating-induced keratinocyte migration. These findings provide important evidence on the role of topographical features of nanofibrous matrices in regulating the phenotypic alteration of keratinocytes and suggest the possible utility of collagen-containing nanofibrous matrices for skin regeneration especially in re-epithelialization.
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影响因子：
14
作者：
Min, BM;Lee, G;Park, WH
通讯作者：
Park, WH
DOI：
10.1016/s0007-1226(52)80004-9
发表时间：
1952-01-01
期刊：
BRITISH JOURNAL OF PLASTIC SURGERY
影响因子：
--
作者：
BILLINGHAM, RE;REYNOLDS, J
通讯作者：
REYNOLDS, J
DOI：
10.1016/s0190-9622(86)70048-0
发表时间：
1986-03-01
影响因子：
13.8
作者：
HEFTON, JM;CALDWELL, D;CARTER, DM
通讯作者：
CARTER, DM
影响因子：
3.7
作者：
Mäkelä, M;Larjava, H;Uitto, VJ
通讯作者：
Uitto, VJ
影响因子：
10.3
作者：
Amano, S;Akutsu, N;Nishiyama, T
通讯作者：
Nishiyama, T