Synthesis of d(GC) and d(CG) octamers containing alternating phosphorothioate linkages: effect of the phosphorothioate group on the B-Z transition.
Synthesis of d(GC) and d(CG) octamers containing alternating phosphorothioate linkages: effect of the phosphorothioate group on the B-Z transition.
复制标题
含有交替硫代磷酸酯键的 d(GC) 和 d(CG) 八聚体的合成：硫代磷酸酯基团对 B-Z 转变的影响。
作者：
R. Cosstick;F. Eckstein
The synthesis of four oligonucleotides containing alternating phosphorothioate groups, (Rp)-and (Sp)-d[G(p(S)CpG)3p(S)C] and (Rp)- and (Sp)-d[C(p(S)GpC)p(S)G], by the phosphite approach is described. Silica gel to which 2'(3')-O-acetyluridine and 5'-succinyl groups were bound served as support for oligomer synthesis. The syntheses were carried out by dimer addition with presynthesized diastereomerically pure dinucleoside phosphorothioates as building blocks. The products were characterized by 31P NMR, nuclease P1 digestion, and oxidation to the corresponding all-phosphate-containing oligomers. The ability of each oligomer to adopt the Z conformation under high-salt conditions was screened for by circular dichroism spectroscopy. Both (Rp)-d[G(p(S)CpG)3p(S)C] and (Sp)-d[C(p(S)GpC)3p(S)G] are capable of forming Z-type structures at high NaCl concentrations. In the case of (Rp)-d[G(p(S)CpG)3p(S)C] where a phosphorothioate of the Rp configuration occurs 5' to a deoxycytidine residue, the B----Z transition is potentiated in comparison to the unmodified oligomer. (Sp)-d[G(p(S)CpG)3p(S)C] and (Rp)-d[C(p(S)GpC)3p(S)G] retain the B conformation even at high NaCl concentration.
双螺旋和三螺旋核酸的磷 31 核磁共振。
影响因子：
2.9
作者：
Gorenstein,DG;Luxon,BA;Goldfield,EM;Lai,K;Vegeais,D
通讯作者：
Vegeais,D
含有胞嘧啶和鸟嘌呤的自互补双链体十四氧核苷酸的构象的序列依赖性。
影响因子：
2.9
作者：
Thomas,GA;Peticolas,WL
通讯作者：
Peticolas,WL