Progesterone improves long-term functional and histological outcomes after permanent stroke in older rats.
Progesterone improves long-term functional and histological outcomes after permanent stroke in older rats.
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DOI：
10.1016/j.bbr.2016.02.024
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发表时间：
2016-05-15
影响因子：
2.7
通讯作者：
Sayeed, Lqbal
中科院分区：
文献类型：
--
作者：
Wali, Bushra;Ishrat, Tauheed;Stein, Donald G.;Sayeed, Lqbal
以前的研究表明，孕酮在中枢神经系统损伤的动物模型中是有益的，但对其在中风后功能恢复方面的长期持续作用知之甚少。我们评估了孕激素对永久性大脑中动脉闭塞（pMCAO）后长达8周的行为测试的影响。雄性Sprague-Dawley大鼠12 m.o.进行pMCAO，并在pMCAO后3小时开始腹腔内注射黄体酮（8 mg/kg）或溶剂，然后在8小时皮下注射，然后每24小时注射一次，持续7天，最后2次治疗逐渐减少。然后在中风后3、6和8周测试大鼠的功能恢复。我们观察到，在中风后8周，经炔雌醇治疗的动物在握力、感觉忽略、运动协调和空间导航测试中显示出梗死体积的减小和功能结果的改善。预后治疗显着改善运动缺陷的患肢的一些步态参数。脑卒中后8周，溶媒组胶质细胞酸性蛋白表达增加，黄体酮组表达显著降低。反复中风后测试，感觉忽略和空间学习表现的某些方面显示出自发恢复，但在步态和握力测量中，仅在中风急性期（前7天）给予孕酮，对所有其他功能恢复的测量显示出持续的有益效果，直到中风后8周。
Previous studies have shown progesterone to be beneficial in animal models of central nervous system injury, but less is known about its longer-term sustained effects on recovery of function following stroke. We evaluated progesterone's effects on a panel of behavioral tests up to 8 weeks after permanent middle cerebral artery occlusion (pMCAO). Male Sprague-Dawley rats 12 m.o. were subjected to pMCAO and, beginning 3 h post-pMCAO, given intraperitoneal injections of progesterone (8 mg/kg) or vehicle, followed by subcutaneous injections at 8 h and then every 24 h for 7 days, with tapering of the last 2 treatments. The rats were then tested on functional recovery at 3, 6 and 8 weeks post-stroke. We observed that progesterone-treated animals showed attenuation of infarct volume and improved functional outcomes at 8 weeks after stroke on grip strength, sensory neglect, motor coordination and spatial navigation tests. Progesterone treatments significantly improved motor deficits in the affected limb on a number of gait parameters. Glial fibrillary acidic protein expression was increased in the vehicle group and considerably lowered in the progesterone group at 8 weeks post-stroke. With repeated post-stroke testing, sensory neglect and some aspects of spatial learning performance showed spontaneous recovery, but on gait and grip-strength measres progesterone given only in the acute stage of stroke (first 7 days) showed sustained beneficial effects on all other measures of functional recovery up to 8 weeks post-stroke.
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影响因子：
3.3
作者：
Ishrat T;Sayeed I;Atif F;Hua F;Stein DG
通讯作者：
Stein DG
影响因子：
8.3
作者：
Coimbra, C;Drake, M;Wieloch, T
通讯作者：
Wieloch, T
影响因子：
1.9
作者：
Freret, Thomas;Bouet, Valentine;Boulouard, Michel
通讯作者：
Boulouard, Michel
影响因子：
6.3
作者：
Hossmann, Konstantin-Alexander
通讯作者：
Hossmann, Konstantin-Alexander
DOI：
10.1097/00004647-199603000-00003
发表时间：
1996-03-01
影响因子：
6.3
作者：
Du, C;Hu, R;Choi, DW
通讯作者：
Choi, DW