Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps.
Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps.
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DOI：
10.1016/j.jaci.2012.02.046
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发表时间：
2012-08
期刊：
影响因子：
--
通讯作者：
Schleimer RP
中科院分区：
文献类型：
--
作者：
Takabayashi T;Kato A;Peters AT;Suh LA;Carter R;Norton J;Grammer LC;Tan BK;Chandra RK;Conley DB;Kern RC;Fujieda S;Schleimer RP
虽然慢性鼻窦炎鼻息肉（CRSwNP）的特点是Th2炎症，肥大细胞的作用知之甚少。本研究的目的是探讨肥大细胞在慢性鼻窦炎（CRS）患者中的存在、定位和表型。我们收集鼻组织和鼻灌洗液从CRS患者和对照组。我们分析了肥大细胞蛋白酶类胰蛋白酶，糜蛋白酶和羧肽酶A3（CPA 3）的mRNA，使用实时PCR，并测量肥大细胞蛋白酶蛋白，ELISA，免疫组织化学和免疫荧光。与CRS患者或对照受试者的钩突组织（UT）相比，CRSwNP患者的鼻息肉（NP）中类胰蛋白酶mRNA显著增加（P <0.001）。类胰蛋白酶蛋白质也在NP和CRSwNP患者的鼻灌洗液中升高。免疫组化结果显示，肥大细胞在上皮和腺体中的数量增加，但在NP的固有层中没有。在NPs上皮中检测到的肥大细胞的特征在于类胰蛋白酶和CPA 3的表达，但不表达糜蛋白酶。肥大细胞表达所有三种蛋白酶丰富的腺上皮内的NP，但没有发现在正常的腺体结构。结论：在这里，我们证明了一个独特的定位肥大细胞内的腺上皮的NP，并显示NP肥大细胞具有不同的表型，不同的组织位置。腺肥大细胞和检测到的肥大细胞的不同亚群可能有助于CRSwNP的发病机制。
Although chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by Th2 inflammation, the role of mast cells is poorly understood. The objective of this study was to investigate the presence, localization and phenotype of mast cells in patients with chronic rhinosinusitis (CRS). We collected nasal tissue and nasal lavage fluid from patients with CRS and control subjects. We analyzed mRNA for the mast cell proteases tryptase, chymase and carboxypeptidase A3 (CPA3), using real-time PCR, and measured mast cell protease proteins using ELISA, immunohistochemistry, and immunofluorescence. Tryptase mRNA was significantly increased in nasal polyps (NPs) from patients with CRSwNP (P < .001) compared with uncinate tissue (UT) from patients with CRS or control subjects. Tryptase protein was also elevated in NPs and in nasal lavage fluids from patients with CRSwNP. Immnohistochemistry showed increased numbers of mast cells in epithelium and glands but not within the lamina propria in NPs. The mast cells detected in epithelium in NPs were characterized by expression of tryptase and CPA3 but not chymase. Mast cells expressing all three proteases were abundant within glandular epithelium of NPs but were not found in normal glandular structures. Conclusion: Herein we demonstrate a unique localization of mast cells within glandular epithelium of NPs, and show that NPs mast cells have distinct phenotypes that vary by tissue location. Glandular mast cells and the diverse subsets of mast cells detected may contribute to the pathogenesis of CRSwNP.
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DOI：
10.1084/jem.20062211
发表时间：
2007-02-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Allakhverdi Z;Comeau MR;Jessup HK;Yoon BR;Brewer A;Chartier S;Paquette N;Ziegler SF;Sarfati M;Delespesse G
通讯作者：
Delespesse G
影响因子：
14.2
作者：
Abonia, J. Pablo;Blanchard, Carine;Butz, Bridget Buckmeier;Rainey, Heather F.;Collins, Margaret H.;Stringer, Keith;Putnam, Philip E.;Rothenberg, Marc E.
通讯作者：
Rothenberg, Marc E.
影响因子：
14.2
作者：
Dougherty, Ryan H.;Sidhu, Sukhvinder S.;Raman, Kavita;Solon, Margaret;Solberg, Owen D.;Caughey, George H.;Woodruff, Prescott G.;Fahy, John V.
通讯作者：
Fahy, John V.
DOI：
10.1038/nri2782
发表时间：
2010-06
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.8
作者：
Choi, Yeon Ho;Lee, Sang-Nam;Yoon, Joo-Heon
通讯作者：
Yoon, Joo-Heon