Reconstruction of rabbit Achilles tendon with three bioabsorbable materials: histological and biomechanical studies

Reconstruction of rabbit Achilles tendon with three bioabsorbable materials: histological and biomechanical studies
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三种生物可吸收材料重建兔跟腱：组织学和生物力学研究

DOI：
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发表时间：
2000
期刊：
Journal of Orthopaedic Science
影响因子：
1.7
通讯作者：
H. Iwase
H. Iwase
中科院分区：
医学4区
文献类型：
--
作者：
Masatoshi Sato;M. Maeda;H. Kurosawa;Y. Inoue;Y. Yamauchi;H. Iwase

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摘要：本研究探讨了聚N-乙酰-D-氨基葡萄糖、聚己内酯(p-CL)、聚乳酸和甲壳素/p-CL三种生物可降解材料在关节外韧带或肌腱重建中作为支架植入材料的可行性。将这些材料制成的编织人工肌腱浸泡在磷酸盐缓冲液中或植入31只日本大白兔的皮下组织中，然后在失败测试时进行加载。甲壳素肌腱在生理盐水中浸泡26周后，在失效时没有明显的负荷损失，但在体内发生了迅速的下降。另外两种类型的肌腱在体外和体内均在26周后显示出明显的强度损失。另取111只兔，用编织肌腱植入物重建跟腱。肌腱再生过程通过宏观、组织学、免疫组织化学和力学方法(失效时的最大负荷)进行评估。甲壳素在组织学检查中表现出比其他材料更快的降解速度。与p-CL纤维相比，甲壳素、聚乳酸和甲壳素/p-CL纤维周围有良好的由I型和III型胶原组成的纤维组织形成，但几丁质肌腱植入后强度下降较快。聚乳酸和甲壳素/p-CL复合肌腱具有良好的初始强度，纤维组织生长增加，有望成为人工肌腱。
Abstract: This study investigated whether three biodegradable materials, poly–N–acetyl–D–glucosamine (chitin), poly-ε-caprolactone (p-CL), polylactic acid (PLA), and chitin/p-CL composite could be used as scaffold implants in the reconstruction of extra-articular ligaments or tendons. Braided artificial tendons made from these materials were soaked in phosphate-buffered saline or implanted in the subcutaneous tissue of 31 Japanese white rabbits and then subjected to load at failure testing. There was no significant loss of load at failure when chitin tendons were soaked in saline for 26 weeks, but a rapid decrease occurred in vivo. The other two types of tendons showed significant loss of strength after 26 weeks both in vitro and in vivo. Another 111 rabbits were used to assess Achilles tendon reconstruction with the braided tendon implants. The tendon regeneration process was assessed macroscopically, histologically, immunohistochemically, and mechanically (maximum load at failure). Chitin showed more rapid degradation than the other materials on histological examination. There was good formation of fibrous tissue composed of type I and type III collagen around the chitin, PLA, and chitin/p-CL fibers in comparison with p-CL fibers, but chitin tendons showed more rapid loss of strength after implantation. Both PLA and the chitin/p-CL composite tendons had good initial strength and showed increased ingrowth of fibrous tissue, suggesting that these materials are promising as artificial tendons.