Rapid microRNA profiling on encoded gel microparticles.
Rapid microRNA profiling on encoded gel microparticles.
复制标题
DOI：
10.1002/anie.201006523
复制
发表时间：
2011-03-01
影响因子：
16.6
通讯作者：
Doyle, Patrick S.
中科院分区：
文献类型：
--
作者：
Chapin, Stephen C.;Appleyard, David C.;Pregibon, Daniel C.;Doyle, Patrick S.
MicroRNA（miRNAs）是介导蛋白质翻译的短的非编码RNA，并且已知在包括糖尿病、阿尔茨海默病和癌症的疾病中失调。[1-3]由于比mRNA具有更高的稳定性和预测价值，这类相对较小的生物分子在确定疾病诊断和预后方面变得越来越重要。然而，序列同源性，广泛的丰度范围，和共同的二级结构的miRNA复杂的努力，以开发准确的，公正的定量技术。[4，5]发现和临床领域的应用需要高通量处理、用于多重分析的大型编码库以及开发定制测定的灵活性。微阵列方法提供高灵敏度和多路复用能力，但其低通量、复杂性和固定设计使其不太适合用于临床环境。[6，7]基于PCR的策略也存在类似的通量问题，但为全基因组miRNA表达谱提供了高度敏感和特异性的检测。[8]替代的基于微珠的系统提供了高的样品通量，但具有降低的灵敏度，[9]动态范围和多路复用能力（luminexcorp. com）。通过深度测序进行的miRNA谱分析正在成为小RNA分析的有力工具;然而，目前实施的高成本和对大量输入RNA的需求限制了其实用性。[10]理想的miRNA定量系统将提供阵列和基于PCR的方法的检测性能，基于珠的系统的通量，并通过用户友好的工作流程提高重现性。我们衷心感谢美国国立卫生研究院和国家癌症研究所的支持。内容完全由作者负责，不一定代表美国国立卫生研究院或美国国立癌症研究所的观点。作者DCP和PSD拥有Firefly BioWorks，Inc.* 的股权。pdoyle@ mit. edu，Homepage：http：//web. mit. edu/doylegroup.
MicroRNAs (miRNAs) are short non-coding RNAs that mediate protein translation and are known to be dysregulated in diseases including diabetes, Alzheimer’s, and cancer.[1–3] With greater stability and predictive value than mRNA, this relatively small class of biomolecules has become increasingly important in determining disease diagnosis and prognosis. However, the sequence homology, wide range of abundance, and common secondary structures of miRNAs have complicated efforts to develop accurate, unbiased quantification techniques.[4, 5] Applications in the discovery and clinical fields require high-throughput processing, large coding libraries for multiplexed analysis, and the flexibility to develop custom assays. Microarray approaches provide high sensitivity and multiplexing capacity, but their low-throughput, complexity, and fixed design make them less than ideal for use in a clinical setting.[6, 7] PCR-based strategies suffer from similar throughput issues, yet offer highly sensitive and specific detection for genome-wide miRNA expression profiling.[8] Alternative bead-based systems provide a high sample throughput, but with reduced sensitivity,[9] dynamic range, and multiplexing capacities (luminexcorp. com). miRNA profiling by deep sequencing is emerging as a powerful tool for small RNA analysis; however, the high cost of implementation and need for large amounts of input RNA currently limit its utility.[10] The ideal system for miRNA quantification would offer the detection performance of array and PCR-based methods, the throughput of bead-based systems, and improved reproducibility with a user-friendly workflow.** We gratefully acknowledge support from the National Institutes of Health and the National Cancer Institute. The content is solely the responsibility of the authors and does not necessarily represent the views of the National Institutes of Health or the National Cancer Institute. The authors DCP and PSD have an equity stake in Firefly BioWorks, Inc.* pdoyle@ mit. edu, Homepage: http://web. mit. edu/doylegroup.
登录
查看更多内容
影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
14.9
作者：
Chen, Jing;Lozach, Jean;Wickham, Eliza;Barnes, Garcia Bret;Luo, Shujun;Mikoulitch, Ivan;Zhou, Lixin;Schroth, Gary;Fan, Jian-Bing
通讯作者：
Fan, Jian-Bing
影响因子：
7.4
作者：
Broder, Graham R.;Ranasinghe, Rohan T.;Morgan, Hywel
通讯作者：
Morgan, Hywel
影响因子：
1.5
作者：
Cheng, Yu-Hung;Chen, Ching-Min;Lee, Ming-Chang M.
通讯作者：
Lee, Ming-Chang M.