Tomato DCL2b is required for the biosynthesis of 22-nt small RNAs, the resulting secondary siRNAs, and the host defense against ToMV.
Tomato DCL2b is required for the biosynthesis of 22-nt small RNAs, the resulting secondary siRNAs, and the host defense against ToMV.
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番茄 DCL2b 是 22-nt 小 RNA 的生物合成、产生的二级 siRNA 以及宿主针对 ToMV 的防御所必需的
DOI：
10.1038/s41438-018-0073-7
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发表时间：
2018
影响因子：
8.7
通讯作者：
Zhu H
中科院分区：
文献类型：
--
作者：
Wang T;Deng Z;Zhang X;Wang H;Wang Y;Liu X;Liu S;Xu F;Li T;Fu D;Zhu B;Luo Y;Zhu H
番茄编码四个功能丰富的DCL家族，其中DCL2家族的研究较少。在这里，我们为番茄DCL2基因dcl2b产生了功能丧失的突变体，并确定了它在防御番茄花叶病毒方面的主要作用，这与自然侵染和人工侵染有关。全基因组小RNA表达谱显示，DCL2b是处理22-NT小RNA所必需的，包括几种miRNAs。有趣的是，这些依赖于DCL2b的22-NT miRNAs的功能类似于DCL1在拟南芥中产生的22-NT miRNAs，并且可以作为触发器来产生一类二级siRNAs。特别是，当植物受到病毒攻击时，大多数次级siRNA来自植物的防御基因。我们还通过RNA-seq检测了dcl2b中差异表达的基因，并观察到在无病毒条件下，许多基因与线粒体代谢和激素信号相关。值得注意的是，当功能丧失的dcl2b突变体受到番茄花叶病毒攻击时，一组防御反应基因被激活，而与脂质代谢相关的基因被抑制。总之，我们的发现为番茄DCL2b在小RNA生物发生和抗病毒防御中的作用提供了新的见解。
The tomato encode four functional DCL families, of which DCL2 is poorly studied. Here, we generated loss-of-function mutants for a tomato DCL2 gene, dcl2b, and we identified its major role in defending against tomato mosaic virus in relation to both natural and manual infections. Genome-wide small RNA expression profiling revealed that DCL2b was required for the processing 22-nt small RNAs, including a few species of miRNAs. Interestingly, these DCL2b-dependent 22-nt miRNAs functioned similarly to the DCL1-produced 22-nt miRNAs in Arabidopsis and could serve as triggers to generate a class of secondary siRNAs. In particular, the majority of secondary siRNAs were derived from plant defense genes when the plants were challenged with viruses. We also examined differentially expressed genes in dcl2b through RNA-seq and observed that numerous genes were associated with mitochondrial metabolism and hormone signaling under virus-free conditions. Notably, when the loss-of-function dcl2b mutant was challenged with tomato mosaic virus, a group of defense response genes was activated, whereas the genes related to lipid metabolism were suppressed. Together, our findings provided new insights into the roles of tomato DCL2b in small RNA biogenesis and in antiviral defense.
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