The Bacillus subtilis DnaD protein: a putative link between DNA remodeling and initiation of DNA replication.

The Bacillus subtilis DnaD protein: a putative link between DNA remodeling and initiation of DNA replication.
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DOI:
10.1016/j.febslet.2004.10.051
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发表时间:
2004-11-19
期刊:
影响因子:
3.5
通讯作者:
Soultanas, P
Soultanas, P
中科院分区:
生物学3区
文献类型:
--
作者:
Turner, IJ;Scott, DJ;Allen, S;Roberts, CJ;Soultanas, P

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枯草芽孢杆菌DNAD蛋白是一种必需的蛋白质,也是ORIC和PriA原始体级联的组成部分,负责将主要的复制环解旋酶DNAC加载到DNA上。我们提供的证据表明,DNAD也具有全球DNA结构活性,在质粒上组装成大的核蛋白复合体,并以类似于最近看到的类似组蛋白的大肠杆菌Hu蛋白的方式对抗质粒紧凑。这种DNA重塑作用可能是在Gram+ve B.subtilis中启动DNA复制的一个重要功能,从而突出了Dna D是细菌类核重组和DNA复制启动之间的联系。
The Bacillus subtilis DnaD protein is an essential protein and a component of the oriC and PriA primosomal cascades, which are responsible for loading the main replicative ring helicase DnaC onto DNA. We present evidence that DnaD also has a global DNA architectural activity, assembling into large nucleoprotein complexes on a plasmid and counteracting plasmid compaction in a manner analogous to that recently seen for the histone-like Escherichia coli HU proteins. This DNA-remodeling role may be an essential function for initiation of DNA replication in the Gram +ve B. subtilis, thus highlighting DnaD as the link between bacterial nucleoid reorganization and initiation of DNA replication.
DOI: 10.1046/j.1365-2958.1997.5951971.x
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