Modulation of the mesolimbic dopaminergic system by beta-endorphin-(1-27) as assessed by microdialysis.
Modulation of the mesolimbic dopaminergic system by beta-endorphin-(1-27) as assessed by microdialysis.
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通过微透析评估β-内啡肽-(1-27) 对中脑边缘多巴胺能系统的调节。
DOI：
10.1016/0014-2999(91)90589-i
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发表时间：
1991
影响因子：
5
通讯作者：
Toni S. Shippenberg
中科院分区：
文献类型：
--
作者：
Rainer Spanagel;Albert Herz;Toni S. Shippenberg
本研究采用在体微透析技术，观察了β-内啡肽（β-EP-（1 - 27））对β-EP（β-EP）诱导的麻醉大鼠延髓核多巴胺（DA）释放的影响。微透析探针插入到核内的多巴胺和peifusates进行了分析，其代谢产物，二羟基苯乙酸（DOPAC）和高香草酸（HVA），使用反相HPLC系统与电化学检测。脑室内（i. c. v.）给予β-EP-（1-27）（5-20 μg）导致DA释放的剂量依赖性增加，其小于β-EP诱导的DA释放，而代谢物水平没有改变。β-EP-（1-27）（5-20 μg）预处理可显著改变β-EP（5 μg）引起的DA释放增加。上述结果表明，β-EP-（1-27）可拮抗β-EP诱导的延髓核DA释放。除了其在β-内啡肽结合位点的拮抗性质外，β-EP-（1-27）似乎是部分激动剂，诱导DA释放增加。这些发现表明，这种天然存在的β-EP片段在中脑边缘系统中具有调节功能。
In the present study we used in vivo microdialysis to examine the influence of β-endorphin-(1-27) (β-EP-(1-27) upon β-endorphin (β-EP)-induced dopamine (DA) release in the nucleus accumbens of anesthetized rats. Microdialysis probes were inserted into the nucleus accumbens and peifusates were analyzed for DA and its metabolites, dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), using a reversed-phase HPLC system with electrochemical detection. Intracerebroventricular (i.c.v.) administration of β-EP-(1-27) (5-20 μg) resulted in a dose-dependent increase in DA release which was smaller than the β-EP-induced DA release, whereas metabolite levels were not altered. Pretreatment with β-EP-(1-27) (5-20 μg) significantly altered the β-EP (5 μg)-induced increase in DA release. These results indicate that β-EP-(1-27) antagonizes the β-EP-induced release of DA in the nucleus accumbens. In addition to its antagonistic properties at the β-endorphin binding site, β-EP-(1-27) appears to be a partial agonist, inducing increased DA release. These findings suggest a regulatory function for this naturally occurring β-EP fragment within the mesolimbic system.
beta-Endorphin-(1-27) 抑制脊髓 β-内啡肽诱导的 Met-脑啡肽释放。
DOI：
10.1111/j.1399-3011.1986.tb01033.x
发表时间：
1986
期刊：
International journal of peptide and protein research
影响因子：
--
作者：
Tseng,LF;Li,CH
通讯作者：
Li,CH