ATPG is required for the accumulation and function of chloroplast ATP synthase in Arabidopsis
ATPG is required for the accumulation and function of chloroplast ATP synthase in Arabidopsis
复制标题
拟南芥叶绿体 ATP 合酶的积累和功能需要 ATPG
DOI：
10.1007/s11434-013-5916-x
复制
发表时间：
2013-06
影响因子：
--
通讯作者：
Mi HuaLing
中科院分区：
文献类型：
--
作者：
Kong MengMeng;Wang FenFei;Yang ZhongNan;Mi HuaLing
叶绿体ATP合酶的亚基II是两个外周柄之一，其将催化CF 1与跨膜CFo相关联。虽然叶绿体ATP合酶的结构和功能已被广泛研究，亚基II在体内的生理意义仍然不清楚。在这项工作中，我们确定了一个拟南芥T-DNA插入突变体的atpG基因编码的叶绿体ATP合酶亚基II。atpg无效突变体显示出白化病致死表型，因为它不能光自养生长。透射电子显微镜分析表明，atpg的叶绿体缺乏有序的类囊体膜。亚基II的缺失影响CF 1-CFo复合物的积累，但似乎对CF 1组装没有影响。与野生型相比，atpg的光诱导ATP形成显著减少。根据这些结果，我们认为ATPG是叶绿体ATP合成酶的积累和功能所必需的。
The subunit II of chloroplast ATP synthase is one of the two peripheral stalks, which associates the catalytic CF1 with membrane-spanning CFo. Although the structural and functional roles of chloroplast ATP synthase have been extensively examined, the physiological significance of subunit II in vivo is still unclear. In this work, we identified one Arabidopsis T-DNA insertion mutant of atpG gene encoding the subunit II of chloroplast ATP synthase. The atpg null mutant displayed an albino lethal phenotype, as it could not grow photoautotrophically. Transmission electron microscopy analysis showed that chloroplasts of atpg lacked the organized thylakoid membranes. Loss of subunit II affected the accumulation of CF1-CFo complex, however, it did not seem to have an effect on the CF1 assembly. The light induced ATP formation of atpg was significantly reduced compared with the wild type. Based on these results, we suggested that ATPG was essential for the accumulation and function of chloroplast ATP synthase.
登录
查看更多内容
影响因子：
3
作者：
R. Priya;V. S. Tadwal;M. Roessle;S. Gayen;C. Hunke;Weng Chuan Peng;J. Torres;G. Grüber
通讯作者：
R. Priya;V. S. Tadwal;M. Roessle;S. Gayen;C. Hunke;Weng Chuan Peng;J. Torres;G. Grüber
影响因子：
44.1
作者：
Xing, Shufan;Miao, Jin;Qu, Li-Jia
通讯作者：
Qu, Li-Jia
DOI：
--
发表时间：
1991-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
N. Inohara;A. Iwamoto;Y. Moriyama;S. Shimomura;M. Maeda;M. Futai
通讯作者：
N. Inohara;A. Iwamoto;Y. Moriyama;S. Shimomura;M. Maeda;M. Futai
DOI：
--
发表时间：
1989-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Lemaire;F. Wollman
通讯作者：
C. Lemaire;F. Wollman
DOI：
10.1515/znc-1999-3-413
发表时间：
1999-04
期刊：
Zeitschrift für Naturforschung C
影响因子：
--
作者：
Hans-Jürgen Tiburzy;M. Zimmermann;R. Oworah-Nkruma;R. Berzborn
通讯作者：
Hans-Jürgen Tiburzy;M. Zimmermann;R. Oworah-Nkruma;R. Berzborn