Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses.
Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses.
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DOI：
10.3390/pharmaceutics14081602
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发表时间：
2022-07-31
期刊：
影响因子：
5.4
通讯作者：
Jang, Mi-Kyeong
中科院分区：
文献类型：
--
作者：
Lee, Jong-Kook;Park, Soyoung;Kim, Young-Min;Guk, Taeuk;Lee, Min-Young;Park, Seong-Cheol;Lee, Jung Ro;Jang, Mi-Kyeong
近年来，耐药真菌的出现加速了新型抗真菌药物的研究。特别是，白色念珠菌感染与医疗器械（如导管、支架和隐形眼镜）上的生物膜形成有关，导致高发病率和死亡率。在这项研究中，我们的目的是阐明一个肽对耐药念珠菌的抗真菌和抗菌膜的作用。白色念珠菌将KWYK序列重复2次和4次，分别得到肽系列1（PS1）-1和PS1-3，并测定了PS1-1、PS1-3和氟康唑对耐药念珠菌的杀灭活性。评估白色念珠菌细胞。PS1-3肽显示出比PS1-1或氟康唑更高的杀伤活性，并且通过膜溶解机制起作用。此外，在接触透镜表面上的最小杀真菌浓度下，PS1-3肽在真菌生物膜抑制和减少方面表现出比PS1-1和氟康唑更有效的活性。总体而言，这些发现确立了PS1-3作为应用于接触镜的潜在杀念珠菌剂。
The recent emergence of antibiotic-resistant fungi has accelerated research on novel antifungal agents. In particular, Candida albicans infections are related to biofilm formation on medical devices, such as catheters, stents, and contact lenses, resulting in high morbidity and mortality. In this study, we aimed to elucidate the antifungal and antibiofilm effects of a peptide against drug-resistant C. albicans. α-Helical peptides in which the sequence of KWYK was repeated twice and four times, designated peptide series 1 (PS1)-1 and PS1-3, respectively, were generated, and the candidacidal activities of PS1-1, PS1-3, and fluconazole against drug-resistant C. albicans cells were assessed. The PS1-3 peptide showed higher killing activity than PS1-1 or fluconazole and acted via a membranolytic mechanism. In addition, the PS1-3 peptide exhibited more potent activity than PS1-1 and fluconazole in terms of fungal biofilm inhibition and reduction at the minimum fungicidal concentration on the contact lens surface. Overall, these findings established PS1-3 as a potential candidacidal agent for applications on contact lenses.
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影响因子：
3.7
作者：
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影响因子：
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