High correlation of the response of upper and lower lobe small airway epithelium to smoking.
High correlation of the response of upper and lower lobe small airway epithelium to smoking.
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DOI：
10.1371/journal.pone.0072669
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Crystal RG
中科院分区：
文献类型：
--
作者：
Harvey BG;Strulovici-Barel Y;Vincent TL;Mezey JG;Raviram R;Gordon C;Salit J;Tilley AE;Chung A;Sanders A;Crystal RG
由吸入香烟烟雾引起的肺部疾病的分布是复杂的，取决于许多因素。小气道上皮细胞（SAE）是吸烟诱导的肺部疾病的最早部位，SAE基因表达可能对吸入的香烟烟雾敏感，我们比较了相同吸烟者SAE中上叶与下叶的基因表达，以确定基因表达模式是否相似或不同。有吸烟诱发性肺病早期证据（肺量正常但弥散能力低）的活跃吸烟者（n =11）接受支气管镜检查和上叶和下叶SAE刷检，以通过微阵列比较上叶与下叶全基因组和吸烟反应基因表达。 聚类和主成分分析表明，对于每个个体，已知的SAE吸烟反应基因的表达在上叶和下叶对中高度相关，尽管正如预期的那样，吸烟诱导的基因表达变化在个体与个体之间存在差异。这些观察结果支持的概念，吸烟者中观察到的吸烟诱导的疾病的解剖分布的异质性不是继发于吸烟对气道上皮细胞的影响的地形差异。
The distribution of lung disease induced by inhaled cigarette smoke is complex, depending on many factors. With the knowledge that the small airway epithelium (SAE) is the earliest site of smoking-induced lung disease, and that the SAE gene expression is likely sensitive to inhaled cigarette smoke, we compared upper vs. lower lobe gene expression in the SAE within the same cigarette smokers to determine if the gene expression patterns were similar or different. Active smokers (n = 11) with early evidence of smoking-induced lung disease (normal spirometry but low diffusing capacity) underwent bronchoscopy and brushing of the upper and lower lobe SAE in order to compare upper vs lower lobe genome-wide and smoking-responsive gene expression by microarray. Cluster and principal component analysis demonstrated that, for each individual, the expression of the known SAE smoking-responsive genes were highly correlated in upper and lower lobe pairs, although, as expected, there were differences in the smoking-induced changes in gene expression from individual to individual. These observations support the concept that the heterogeneity observed among smokers in the anatomic distribution of smoking-induced disease are not secondary to the topographic differences in the effects of cigarette smoke on the airway epithelium.
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影响因子：
158.5
作者：
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通讯作者：
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影响因子：
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