Differentiation of serum-free embryoid bodies from human induced pluripotent stem cells into networks.
Differentiation of serum-free embryoid bodies from human induced pluripotent stem cells into networks.
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来自人类诱导多能干细胞的无血清胚状体分化成网络。
DOI：
10.1016/j.scr.2013.02.001
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发表时间：
2013
影响因子：
1.2
通讯作者：
Noggle,ScottA
中科院分区：
文献类型：
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作者：
Nestor,MichaelW;Paull,Daniel;Jacob,Samson;Sproul,AndrewA;Alsaffar,Abdulaziz;Campos,BrianA;Noggle,ScottA
Three-dimensional aggregation cultures allow for complex development of differentiated human induced pluripotent stem cells. However, this approach is not easily amenable to live-cell imaging and electrophysiological applications due to the thickness and the geometry of the tissue. Here, we present an improvement on the traditional aggregation method by combining the use of cell culture inserts with serum-free embryoid bodies (SFEBs). The use of this technique allows the structures to maintain their three-dimensional structure while thinning substantially. We demonstrate that this technique can be used for electrophysiological recodings as well as live-cell calcium imaging combined with electrical stimulation, akin to organotypic slice preparations. This provides an important experimental tool that can be used to bridge 3-D structures with traditional monolayer approaches used in stem cell applications.
第 5 章 – 使用脑切片培养物研究癫痫
DOI：
--
发表时间：
2006
期刊：
影响因子：
--
作者：
S. Thompson;Xiang Cai;C. Dinocourt;Michael W. Nestor
通讯作者：
Michael W. Nestor
自发同步网络活动激活早期沉默突触限制了新皮质连接的范围
影响因子：
5.3
作者：
T. Voigt;T. Opitz;A. D. de Lima
通讯作者：
A. D. de Lima