A novel strategy to analyze L-tryptophan through allosteric Trp repressor based on rolling circle amplification.
A novel strategy to analyze L-tryptophan through allosteric Trp repressor based on rolling circle amplification.
复制标题
基于滚环扩增的变构色氨酸阻遏物分析 L-色氨酸的新策略。
DOI：
10.1016/j.bios.2015.04.017
复制
发表时间：
2015-09
影响因子：
12.6
通讯作者：
Guan Yifu
中科院分区：
文献类型：
--
作者：
Zhao Guojie;Hu Tianyu;Li Jun;Wei Hua;Shang Hong;Guan Yifu
Rolling circle amplification (RCA) has been considered as a powerful tool for nucleic acids detection. Here, a novel repressor-RCA-based method forl-tryptophan (l-Trp) detection was developed. This method utilizes the specific interaction between the RCA circular template and the Trp repressor protein (TrpR) involved intrpoperon ofEscherichia coli(E. coli). In the absence ofl-Trp, the TrpR protein could not bind to the RCA template, and the RCA process can be continued. Whenl-Trp is present, the activated TrpR will bind to the operon sequence on the RCA template and inhibit the RCA reaction. Thus, the concentration ofl-Trp is correlated directly with the fluorescent RCA signals. We succeeded in detectingl-Trp in a single step in simple homogeneous reaction system. The detection limit was estimated to be 0.77 μM (S/N=3) with good linearity. The method can unambiguously distinguishl-Trp from other 19 standard amino acids andl-Trp analogs. This strategy is also promising for detecting many small molecules such as other amino acids and carbohydrates.
登录
查看更多内容
影响因子：
4.9
作者：
Qingwang Xue;Lei Wang;Wei Jiang
通讯作者：
Qingwang Xue;Lei Wang;Wei Jiang
使用适体引发的滚环扩增进行蛋白质超灵敏检测的简便扫描测量策略
影响因子：
4.9
作者：
Cheng, Wei;Ding, Lin;Yin, Yibing
通讯作者：
Yin, Yibing
影响因子：
4.9
作者：
Guojie Zhao;Tianyu Hu;Jun Li;Hua Wei;H. Shang;Y. Guan
通讯作者：
Guojie Zhao;Tianyu Hu;Jun Li;Hua Wei;H. Shang;Y. Guan
影响因子：
12.6
作者：
Shurong Tang;P. Tong;Heng-Dong Li;Jing-Xiao Tang;Lan Zhang
通讯作者：
Shurong Tang;P. Tong;Heng-Dong Li;Jing-Xiao Tang;Lan Zhang
影响因子：
15
作者：
Cho, EJ;Yang, LT;Ellington, AD
通讯作者：
Ellington, AD