Differential regulation of inositol 1,4,5‐trisphosphate by co‐existing P2Y‐purinoceptors and nucleotide receptors on bovine aortic endothelial cells
Differential regulation of inositol 1,4,5‐trisphosphate by co‐existing P2Y‐purinoceptors and nucleotide receptors on bovine aortic endothelial cells
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牛主动脉内皮细胞上共存的 P2Y 嘌呤受体和核苷酸受体对肌醇 1,4,5-三磷酸的差异调节
DOI：
10.1111/j.1476-5381.1994.tb14797.x
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发表时间：
1994
影响因子：
7.3
通讯作者：
M. Boarder
中科院分区：
文献类型：
--
作者：
J. Purkiss;G. Wilkinson;M. Boarder
1我们检测了牛主动脉内皮细胞（BAE）对嘌呤（ATP、ADP及其类似物）和嘧啶、尿苷三磷酸（UTP）的1，4，5-三磷酸肌醇（Ins（1，4，5）P3）反应。2在无激动剂的情况下，BAE细胞上的培养基交换被发现是对Ins（1，4，5）P3产生的刺激。用100 μm ATP、30 μm 2 MeSATP（P2 Y-嘌呤受体激动剂，但不是核苷酸受体激动剂）或100 μm UTP（核苷酸受体激动剂，但不是P2 Y-嘌呤受体激动剂）刺激的BAE细胞产生的Ins（1，4，5）P3反应高于更换培养基引起的反应。时间过程是快速的，在前5秒内达到峰值反应，刺激30秒后水平恢复接近基础。3 Ins（1，4，5）P3对100 μ mUTP和30 μ m2MeSATP刺激的反应有显著差异。对UTP的反应比对2 MeSATP的反应更持久。4用100 μ mUTP加30 μ m2MeSATP刺激BAE细胞，产生的反应与单独加入两种激动剂的反应在统计学上没有区别。5通过用百日咳毒素预处理BAE细胞，Ins（1，4，5）P3对UTP的反应减弱至对照组的25%。对2 MeSATP和ADP的反应基本上不受影响。ATP刺激减少到对照的65%。6用十四酰佛波醇乙酸酯（TPA）激活蛋白激酶C，可显著抑制Ins（1，4，5）P3对2 MeSATP和ADP的反应，但对UTP刺激无影响。蛋白激酶C抑制剂Ro 31-8220可增强对2 MeSATP、ADP和ATP的反应，但对UTP刺激无影响。这些观察结果表明，核苷酸和P2 Y受体通过不同的途径动员第二信使Ins（1，4，5）P3，导致不同的生成模式，并表明虽然ATP激活两种受体，但ADP主要通过与P2 Y嘌呤受体相互作用来影响这些细胞。
1 We have examined the inositol 1,4,5‐trisphosphate (Ins(1,4,5)P3) responses in bovine aortic endothelial (BAE) cells to purines (ATP, ADP and analogues) and the pyrimidine, uridine triphosphate (UTP). 2 Exchange of medium on BAE cells in the absence of agonist was found to be a stimulus for Ins(1,4,5)P3 generation. BAE cells stimulated with 100 μm ATP, 30 μm 2MeSATP (an agonist at P2Y‐purinoceptors but not nucleotide receptors) or 100 μm UTP (an agonist at nucleotide receptors but not P2Y‐purinoceptors) gave Ins(1,4,5)P3 responses above that caused by exchange of medium. The time course was rapid, with peak response within the first 5 s and levels returning close to basal after 30 s of stimulation. 3 Significant differences in Ins(1,4,5)P3 responses to 100 μm UTP and 30 μm 2MeSATP stimulation were observed. The response to UTP was reproducibly more sustained than that to 2MeSATP. 4 Stimulation of BAE cells with 100 μm UTP plus 30 μm 2MeSATP produced a response statistically indistinguishable from that predicted by addition of the responses to the two agonists in isolation. 5 The Ins(1,4,5)P3 response to UTP was attenuated to 25% of control by pretreatment of BAE cells with pertussis toxin. Responses to 2MeSATP and ADP were essentially unaffected. ATP stimulation was reduced to 65% of control. 6 Activation of protein kinase C with tetradecanoyl phorbol acetate (TPA) profoundly inhibited Ins(1,4,5)P3 responses to 2MeSATP and ADP but had no effect on UTP stimulation. The protein kinase C inhibitor, Ro 31–8220, enhanced responses to 2MeSATP, ADP and ATP but no effect was observed on UTP stimulation. 7 These observations show that nucleotide and P2Y‐receptors mobilise the second messenger Ins (1,4,5)P3 by separate routes resulting in different patterns of generation and suggest that while ATP activates both receptors, ADP principally influences these cells by interacting with the P2Y‐purinoceptors.
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影响因子：
56.9
作者：
SMRCKA, AV;HEPLER, JR;STERNWEIS, PC
通讯作者：
STERNWEIS, PC
有证据表明 UTP 和 ATP 通过人气道上皮细胞中常见的胞外 5-核苷酸受体调节磷脂酶 C。
影响因子：
3.6
作者：
Brown,HA;Lazarowski,ER;Boucher,RC;Harden,TK
通讯作者：
Harden,TK
使用纯化的 m1 毒蕈碱受体、Gq/11 和磷脂酶 C-β 1 重建激动剂刺激的磷脂酰肌醇 4,5-二磷酸水解。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Berstein,G;Blank,JL;Smrcka,AV;Higashijima,T;Sternweis,PC;Exton,JH;Ross,EM
通讯作者：
Ross,EM
犬血管内皮和平滑肌的不同 P2 嘌呤能受体亚型。
DOI：
--
发表时间：
1987
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Houston,DA;Burnstock,G;Vanhoutte,PM
通讯作者：
Vanhoutte,PM
长期佛波酯治疗可下调蛋白激酶 C 并使磷酸肌醇信号通路对激素和生长因子刺激敏感。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hepler,JR;Earp,HS;Harden,TK
通讯作者：
Harden,TK