Getting sweeter: new evidence for glucose transporters in specific cell types of the airway?
Getting sweeter: new evidence for glucose transporters in specific cell types of the airway?
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DOI：
10.1152/ajpcell.00140.2022
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发表时间：
2023-01-01
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影响因子：
--
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单细胞RNA测序(ScRNAseq)等新技术使识别单个细胞表达的mRNA转录本成为可能。这篇综述提供了最近对从气管到肺泡的呼吸道上皮细胞中葡萄糖转运蛋白表达的scRNAseq研究的洞察力。分析的研究数量有限，并不是所有的研究都报道了葡萄糖转运蛋白的全部范围，而且新鲜从呼吸道分离的细胞与体外培养的细胞之间存在差异。此外，葡萄糖转运体基因转录本的表达水平低于其他上皮标志物基因。然而，这些研究强调了葡萄糖转运蛋白在细胞中的表达存在差异。Glut1是广泛表达的转运蛋白中含量最丰富的，包括GLUT8、10和13。GLUT9转录本在基底细胞中更常见，GLUT12转录本在离子细胞/纤毛细胞中更常见。除肺泡细胞外，SGLT1转录本还存在于分泌细胞中。GLUT3mRNA转录本在表达单羧酸(MCT2)转运蛋白的细胞团中表达。这些分布可能是支持细胞增殖、离子转运、粘液分泌、环境感知和呼吸道葡萄糖动态平衡的细胞特异性代谢需求的基础。这些研究还强调了葡萄糖转运体在脱氢抗坏血酸/维生素C/肌醇/尿酸盐运动中的作用，这些因素对呼吸道的先天免疫特性很重要。在检测肺中的mRNAs、蛋白质和葡萄糖转运蛋白的功能之间仍然存在差异。然而，对来自进一步scRNAseq研究的数据进行整理可能会提供更好的共识和理解，并得到qPCR、免疫组织化学和功能实验的支持。
New technologies such as single-cell RNA sequencing (scRNAseq) has enabled identification of the mRNA transcripts expressed by individual cells. This review provides insight from recent scRNAseq studies on the expression of glucose transporters in the epithelial cells of the airway epithelium from trachea to alveolus. The number of studies analyzed was limited, not all reported the full range of glucose transporters and there were differences between cells freshly isolated from the airways and those grown in vitro. Furthermore, glucose transporter mRNA transcripts were expressed at lower levels than other epithelial marker genes. Nevertheless, these studies highlighted that there were differences in cellular expression of glucose transporters. GLUT1 was the most abundant of the broadly expressed transporters that included GLUT8, 10, and 13. GLUT9 transcripts were more common in basal cells and GLUT12 in ionocytes/ciliated cells. In addition to alveolar cells, SGLT1 transcripts were present in secretory cells. GLUT3 mRNA transcripts were expressed in a cell cluster that expressed monocarboxylate (MCT2) transporters. Such distributions likely underlie cell-specific metabolic requirements to support proliferation, ion transport, mucous secretion, environment sensing, and airway glucose homeostasis. These studies have also highlighted the role of glucose transporters in the movement of dehydroascorbic acid/vitamin C/myoinositol/urate, which are factors important to the innate immune properties of the airways. Discrepancies remain between detection of mRNAs, protein, and function of glucose transporters in the lungs. However, collation of the data from further scRNAseq studies may provide a better consensus and understanding, supported by qPCR, immunohistochemistry, and functional experiments.
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影响因子：
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影响因子：
24.7
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