Quantification of Microbial Communities in Forearc Sediment Basins off Sumatra
Quantification of Microbial Communities in Forearc Sediment Basins off Sumatra
复制标题
苏门答腊岛弧前沉积盆地微生物群落的量化
DOI：
10.1080/01490450903456798
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发表时间：
2010
影响因子：
2.3
通讯作者：
B. Teichert
中科院分区：
文献类型：
--
作者：
A. Schippers;Gerrit Köweker;Carmen Höft;B. Teichert
在印度尼西亚苏门答腊岛海岸外的印度洋沉积物中，在沉积物表面附近的25个高分辨率采样站和在海底以下最大深度12米（mbsf）的3个站点进行了定量微生物群落分析。以SYBR Green II作为荧光染色染料，采用两种不同的方法测定细胞总数。在不与沉积物颗粒分离的情况下，沉积物表面的总细胞计数为10 9个细胞/ mL沉积物，各站之间差异不大。在0.2 ~ 0.4 mbsf时降至10 8个细胞/ mL，在6 mbsf以下降至10 7个细胞/ mL。从沉积物颗粒中分离细胞后，细胞总数在6 mbsf以上下降了一个数量级，下图显示了类似的值。这两种方案的差异可以解释为在分离过程中细胞的损失和/或在没有从沉积物颗粒中分离细胞的情况下计数非特异性信号。以16S rRNA和功能基因为靶点，采用实时荧光定量PCR （Q-PCR）技术对特定的系统发育和生理原核群体进行定量分析。在所有沉积物深度的总细胞数范围内，古菌和细菌的16S rRNA基因拷贝数总体上相似，因此古菌和细菌都不能被认为是优势菌。真核生物的18S rRNA基因数量比原核生物的16S rRNA基因少两个数量级。在不同的（高达108个基因拷贝/ mL）沉积物中检测到Fe(III)-和Mn(IV)-还原细菌（Geobacteraceae的16S rRNA基因）和硫酸盐还原细菌（功能基因dsrA），但其数量均显著低于细菌总数。硫酸盐还原剂在原核生物群落中的比例为0.2 ~ 19%。计算出的空气硫酸盐还原率总体上较低，在0.002至0.027 mmol m−2 a−1之间，导致每个细胞的硫酸盐还原率为0.0007至0.81 fmol cell−1 a−1，与其他深埋海洋沉积物的已发表数据相似。甲烷生成似乎没有发挥很大的作用，因为甲烷仅在6.5 mbsf以下被检测到，并且在任何样品中都无法检测到产甲烷菌和厌氧产甲烷菌的功能基因mcrA。
Sediments in the Indian Ocean off the coast of the Indonesian island Sumatra were sampled at 25 stations in high resolution near the sediment surface and at three stations up to a maximum depth of 12 meter below seafloor (mbsf) for a quantitative microbial community analysis. Total cell counts were determined applying two different protocols including SYBR Green II as fluorescent staining dye. Total cell counts without detaching cells from sediment particles were 10 9 cells/ mL sediments at the sediment surface with little variation between all stations. They decreased to 10 8 cells/ mL at 0.2 to 0.4 mbsf and to 10 7 cells/ mL below 6 mbsf. The total cell counts after detaching cells from sediment particles were up to one order of magnitude lower above 6 mbsf and showed similar values below. This difference for the two protocols can be explained by a loss of cells during the detachment procedure and/or counting of unspecific signals without detaching cells from sediment particles. Particular phylogenetic and physiological prokaryotic groups were quantified by quantitative, real-time PCR (Q-PCR) targeting 16S rRNA and functional genes. Archaea and Bacteria were found overall in similar 16S rRNA gene copy numbers in the range of the total cell counts at all sediment depths, thus, neither Archaea nor Bacteria could be considered as dominant. The eukaryotic 18S rRNA gene occurred in two orders of magnitude lower numbers than prokaryotic 16S rRNA genes. Fe(III)- and Mn(IV)-reducing bacteria (16S rRNA gene of Geobacteraceae) and sulfate-reducing bacteria (functional gene dsrA) were detected in variable (up to 10 8 gene copies/ mL sediment) but in always significantly lower numbers than total Bacteria. The proportion of sulfate reducers on the prokaryotic community was between 0.2 and 19%. Calculated aereal sulfate reduction rates were overall low with values between 0.002 and 0.027 mmol m − 2 a − 1 , resulting in sulfate reduction rates per cell of 0.0007 and 0.81 fmol cell − 1 a − 1 , similar to published data for other deeply buried marine sediments. Methanogenesis did not seem to play a big role since methane was detected only below 6.5 mbsf, and the functional gene of methanogens and anaerobic methanotrophs mcrA could not be detected in any sample.
影响因子：
5.1
作者：
Webster G;Blazejak A;Cragg BA;Schippers A;Sass H;Rinna J;Tang X;Mathes F;Ferdelman TG;Fry JC;Weightman AJ;Parkes RJ
通讯作者：
Parkes RJ
影响因子：
5
作者：
Hensen, C;Zabel, M;Boettius, A
通讯作者：
Boettius, A