Subsurface microbiology and biogeochemistry of a deep, cold-water carbonate mound from the Porcupine Seabight (IODP Expedition 307).
Subsurface microbiology and biogeochemistry of a deep, cold-water carbonate mound from the Porcupine Seabight (IODP Expedition 307).
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DOI：
10.1111/j.1462-2920.2008.01759.x
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发表时间：
2009-01
影响因子：
5.1
通讯作者：
Parkes RJ
中科院分区：
文献类型：
--
作者：
Webster G;Blazejak A;Cragg BA;Schippers A;Sass H;Rinna J;Tang X;Mathes F;Ferdelman TG;Fry JC;Weightman AJ;Parkes RJ
Porcupine Seabight Challenger Mound是第一个钻探的碳酸盐岩丘（270米），并进行了详细的微生物和地球化学分析。采用一系列分子技术[催化报告沉积-荧光原位杂交（CARD-FISH）、定量PCR（16 S rRNA和功能基因，dsrA和mcrA）以及16 S rRNA基因PCR-DGGE]分析了两个丘位点和一个非丘参考位点，以评估原核生物多样性，并将其与总细胞和可培养细胞计数、放射性示踪剂活性测量和地球化学的分布进行比较。在碳酸盐丘内部和下方都有一个重要而活跃的原核生物群落。虽然某些深度的细胞总数低于其他海底下沉积物的全球平均数，而且原核生物的活动也相对较低（铁和硫酸盐还原、乙酸盐氧化、甲烷生成），但与参考地点相比，这些细胞总数显著增加。此外，在最深的沉积物（中新世，> 10 Ma）中有一些原核生物活动的刺激，包括土墩底部以下甲烷活动的厌氧氧化潜力。细菌和古细菌都存在，但两者都不占优势，它们与其他海底下沉积物中常见的序列有关。据估计，仅在豪猪盆地就有大约1600个土丘，碳酸盐土丘可能是重要的原核生物海底下栖息地。
The Porcupine Seabight Challenger Mound is the first carbonate mound to be drilled (∼270 m) and analyzed in detail microbiologically and biogeochemically. Two mound sites and a non-mound Reference site were analyzed with a range of molecular techniques [catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH), quantitative PCR (16S rRNA and functional genes, dsrA and mcrA), and 16S rRNA gene PCR-DGGE] to assess prokaryotic diversity, and this was compared with the distribution of total and culturable cell counts, radiotracer activity measurements and geochemistry. There was a significant and active prokaryotic community both within and beneath the carbonate mound. Although total cell numbers at certain depths were lower than the global average for other subseafloor sediments and prokaryotic activities were relatively low (iron and sulfate reduction, acetate oxidation, methanogenesis) they were significantly enhanced compared with the Reference site. In addition, there was some stimulation of prokaryotic activity in the deepest sediments (Miocene, > 10 Ma) including potential for anaerobic oxidation of methane activity below the mound base. Both Bacteria and Archaea were present, with neither dominant, and these were related to sequences commonly found in other subseafloor sediments. With an estimate of some 1600 mounds in the Porcupine Basin alone, carbonate mounds may represent a significant prokaryotic subseafloor habitat.
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影响因子：
4.4
作者：
Holmes, DE;Finneran, KT;Lovley, DR
通讯作者：
Lovley, DR
DOI：
10.1073/pnas.0600035103
发表时间：
2006-03-07
影响因子：
11.1
作者：
Biddle, JF;Lipp, JS;Hinrichs, KU
通讯作者：
Hinrichs, KU
影响因子：
56.9
作者：
D'Hondt, S;Jorgensen, BB;Acosta, JLS
通讯作者：
Acosta, JLS
影响因子：
64.8
作者：
Boetius, A;Ravenschlag, K;Pfannkuche, O
通讯作者：
Pfannkuche, O
影响因子：
5.8
作者：
Kano, Akihiro;Ferdelman, Timothy G.;Li, Xianghui
通讯作者：
Li, Xianghui