Distinct tooth regeneration systems deploy a conserved battery of genes.
Distinct tooth regeneration systems deploy a conserved battery of genes.
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DOI：
10.1186/s13227-021-00172-3
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发表时间：
2021-03-25
期刊：
影响因子：
4.1
通讯作者：
Miller CT
中科院分区：
文献类型：
--
作者：
Square TA;Sundaram S;Mackey EJ;Miller CT
脊椎动物的牙齿表现出广泛的再生系统。许多物种，包括大多数哺乳动物、爬行动物和两栖动物，在组织学上不同的位置（称为连续牙板）形成替换牙齿，而其他物种则不采用这样的系统。值得注意的是，“无椎板”牙齿替换情况存在于并系系的射线鳍鱼类中，例如刺鱼、鳟鱼、鳕鱼、青鳉和比齐尔鱼。此外，不同脊椎动物牙齿再生系统的位置、更新潜力和潜伏时间似乎存在很大差异。因此，牙齿再生的祖细胞呈现出高度不同的排列和潜力。考虑到脊椎动物中存在的再生系统的范围，目前尚不清楚形态上不同的牙齿再生系统是否在其原始牙组织中部署了一组重叠的基因。在目前的工作中，我们的目的是确定牙齿祖上皮是否可以由具有不同再生系统的脊椎动物牙列之间的保守细胞类型组成。为了解决这个问题，我们比较了两种射线鳍鱼类的咽齿再生过程：斑马鱼（Danio rerio）和三刺刺鱼（Gasterosteus aculeatus）。这两种硬骨鱼在大约 2.5 亿年前分化，在牙齿形态和再生方面表现出一些明显的差异。在这里，我们发现斑马鱼的幼稚连续牙板表达一组九个基因（bmpr1aa、bmp6、cd34、gli1、igfbp5a、lgr4、lgr6、nfatc1和pitx2），而活跃的Wnt信号传导和Lef1表达发生在牙齿发育的早期形态发生阶段。我们还发现，尽管刺鱼牙区不存在组织学上不同的连续牙板，但相同的九个基因组（Bmpr1a、Bmp6、CD34、Gli1、Igfbp5a、Lgr4、Lgr6、Nfatc1 和 Pitx2）在最基底内胚层细胞层中表达，这是与替换牙胚关系最密切的区域。与斑马鱼一样，刺鱼替代牙胚也表达 Lef1 并表现出活跃的 Wnt 信号传导。因此，两种鱼类系统要么具有有组织的连续牙板（斑马鱼），要么缺乏形态上不同的连续牙板（棘鱼），在牙齿再生过程中部署了相似的遗传程序。我们建议此处描述的表达域描绘了高度保守的“连续牙上皮”（SDE）。此外，已知一组直系同源基因可标记小鼠的毛囊上皮干细胞，这表明其他上皮附属物的再生系统可能利用相关的上皮祖细胞类型，尽管所得功能器官具有高度衍生的性质。
Vertebrate teeth exhibit a wide range of regenerative systems. Many species, including most mammals, reptiles, and amphibians, form replacement teeth at a histologically distinct location called the successional dental lamina, while other species do not employ such a system. Notably, a ‘lamina-less’ tooth replacement condition is found in a paraphyletic array of ray-finned fishes, such as stickleback, trout, cod, medaka, and bichir. Furthermore, the position, renewal potential, and latency times appear to vary drastically across different vertebrate tooth regeneration systems. The progenitor cells underlying tooth regeneration thus present highly divergent arrangements and potentials. Given the spectrum of regeneration systems present in vertebrates, it is unclear if morphologically divergent tooth regeneration systems deploy an overlapping battery of genes in their naïve dental tissues. In the present work, we aimed to determine whether or not tooth progenitor epithelia could be composed of a conserved cell type between vertebrate dentitions with divergent regeneration systems. To address this question, we compared the pharyngeal tooth regeneration processes in two ray-finned fishes: zebrafish (Danio rerio) and threespine stickleback (Gasterosteus aculeatus). These two teleost species diverged approximately 250 million years ago and demonstrate some stark differences in dental morphology and regeneration. Here, we find that the naïve successional dental lamina in zebrafish expresses a battery of nine genes (bmpr1aa, bmp6, cd34, gli1, igfbp5a, lgr4, lgr6, nfatc1, and pitx2), while active Wnt signaling and Lef1 expression occur during early morphogenesis stages of tooth development. We also find that, despite the absence of a histologically distinct successional dental lamina in stickleback tooth fields, the same battery of nine genes (Bmpr1a, Bmp6, CD34, Gli1, Igfbp5a, Lgr4, Lgr6, Nfatc1, and Pitx2) are expressed in the basalmost endodermal cell layer, which is the region most closely associated with replacement tooth germs. Like zebrafish, stickleback replacement tooth germs additionally express Lef1 and exhibit active Wnt signaling. Thus, two fish systems that either have an organized successional dental lamina (zebrafish) or lack a morphologically distinct successional dental lamina (sticklebacks) deploy similar genetic programs during tooth regeneration. We propose that the expression domains described here delineate a highly conserved “successional dental epithelium” (SDE). Furthermore, a set of orthologous genes is known to mark hair follicle epithelial stem cells in mice, suggesting that regenerative systems in other epithelial appendages may utilize a related epithelial progenitor cell type, despite the highly derived nature of the resulting functional organs.
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影响因子：
23.9
作者：
Brownell, Isaac;Guevara, Elizabeth;Bai, C. Brian;Loomis, Cynthia A.;Joyner, Alexandra L.
通讯作者：
Joyner, Alexandra L.
影响因子：
15.9
作者：
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通讯作者：
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DOI：
10.1073/pnas.1407567111
发表时间：
2014-09-23
影响因子：
11.1
作者：
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通讯作者：
Miller, Craig T.
影响因子：
1.5
作者：
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通讯作者：
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影响因子：
7.7
作者：
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通讯作者：
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