Imaging of the cytoplasmic leaflet of the plasma membrane by atomic force microscopy.
Imaging of the cytoplasmic leaflet of the plasma membrane by atomic force microscopy.
复制标题
通过原子力显微镜对质膜的细胞质小叶进行成像。
DOI：
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
J. Goudonnet
中科院分区：
文献类型：
--
作者：
C. LeGrimellec;Eric Lesniewska;M. Giocondi;C. Cachia;J. Schreiber;J. Goudonnet
用原子力显微镜(AFM)在空气和水介质中以“接触”的方式对生长在玻片上的马丁达比犬肾(MDCK)细胞腹侧细胞膜的细胞质表面进行成像。对风干样品的微米扫描显示出一种异质结构，其中有一些细丝，可能对应于靠近膜内小叶的肌动蛋白细丝，以及一些半组织的晶格结构，可能对应于笼状晶格。在磷酸盐缓冲盐水中的实验证实了内膜表面的异质性，从表面出现了大的(>；100 nm)球状结构。利用亚微米扫描范围，在液体介质和空气中对占据膜表面大部分的突出颗粒进行成像。这些颗粒在乙醇脱水后仍然存在，大小为8到40 nm x-y，提取了很大一部分膜脂，表明它们具有蛋白质性质。至少部分由于某些外周蛋白的存在，内膜表面的高倍率图像在颗粒分布方面是不均匀的。这些数据与之前报道的活的MDCK细胞表面的外膜小叶的数据进行了比较。它们表明，细胞质膜表面的细节可以用原子力显微镜来分辨。最后，这些图像支持了蛋白质接近的质膜组织的观点。
The cytoplasmic face of ventral cell membranes of Madin-Darby canine kidney (MDCK) cells grown on glass coverslips was imaged by atomic force microscopy (AFM) in air and under aqueous medium, in "contact" mode. Micrometer range scans on air-dried samples revealed a heterogeneous structure with some filaments, likely corresponding to actin filaments that abut the inner leaflet of the membrane, and a few semi-organized lattice structures that might correspond to clathrin lattices. Experiments in phosphate-buffered saline confirmed the heterogeneity of the inner membrane surface with the presence of large (> 100 nm) globular structures emerging from the surface. Using sub-micrometer scan ranges, protruding particles, that occupy most of the membrane surface, were imaged in liquid medium and in air. These particles, 8 to 40 nm x-y size, were still present following ethanol dehydration which extracts a large fraction of membrane lipids, indicating their proteic nature. Due, at least partly, to the presence of some peripheral proteins, high magnification images of the inner membrane surface were heterogeneous with regard to particle distribution. These data compare with those previously reported for the external membrane leaflet at the surface of living MDCK cells. They show that details of the cytosolic membrane surface can be resolved by AFM. Finally, the images support the view of a plasma membrane organization where proteins come into close proximity.
影响因子：
2.2
作者：
VESENKA, J;GUTHOLD, M;BUSTAMANTE, C
通讯作者：
BUSTAMANTE, C