Effects of Y27632 on keratinocyte procurement and wound healing.
Effects of Y27632 on keratinocyte procurement and wound healing.
复制标题
DOI：
10.1111/ced.12067
复制
发表时间：
2013-10
影响因子：
4.1
通讯作者：
Zhang JY
中科院分区：
文献类型：
--
作者：
Gandham VD;Maddala RL;Rao V;Jin JY;Epstein DL;Hall RP;Zhang JY
已经开发了许多Rho激酶抑制剂用于各种临床应用。我们研究了Rho激酶抑制剂Y27632对角质形成细胞增殖和迁移的影响，发现它促进了单层和皮肤外植体培养中的原代人角质形成细胞增殖和迁移。此外，局部应用Y27632增强了小鼠大多数伤口的皮肤伤口闭合。与真皮成纤维细胞相比，Y27632的生长和迁移作用似乎对角质形成细胞具有特异性，并且需要完整的Jun激酶功能。Y27632似乎是一种有前途的角质形成细胞采购和创伤愈合剂。已经开发了许多Rho信号级联的药理学抑制剂用于生物学和临床应用。其中之一，Y27632，一种Rho相关蛋白激酶（ROCK）的抑制剂，已被证明可以增加胚胎干细胞的存活率和培养中的角质形成细胞干细胞的获得。本研究的目的是检查Y27632如何影响角质形成细胞和真皮成纤维细胞的生长和迁移，并评估该试剂对皮肤伤口愈合的体内作用。
A number of Rho-kinase inhibitors have been developed for various clinical applications. We examined the effects of the Rho-kinase inhibitor Y27632 on keratinocyte proliferation and migration, and found it promoted primary human keratinocyte proliferation and migration in both monolayer and skin explant cultures. In addition, topical application of Y27632 enhanced cutaneous wound closure in the majority of wounds in mice. The growth and migration effects of Y27632 appeared to be specific to keratinocytes compared with dermal fibroblasts, and required intact Jun kinase function. Y27632 seems to be a promising agent for keratinocyte procurement and wounding healing. A number of pharmacological inhibitors of the Rho signalling cascade have been developed for biological and clinical applications. One of these, Y27632, an inhibitor of Rho-associated protein kinase (ROCK), has been shown to increase embryonic stem-cell survival and keratinocyte stem-cell procurement in culture. The aim of this study was to examine how Y27632 affects keratinocyte and dermal fibroblast cell growth and migration, and to evaluate the in vivo effects of this reagent on cutaneous wound healing.
登录
查看更多内容
影响因子：
2.9
作者：
Han, YP;Nien, YD;Garner, WL
通讯作者：
Garner, WL
影响因子：
64.8
作者：
Uehata, M;Ishizaki, T;Narumiya, S
通讯作者：
Narumiya, S
影响因子：
4.1
作者：
Terunuma, Atsushi;Limgala, Renuka Pudi;Vogel, Jonathan C.
通讯作者：
Vogel, Jonathan C.
影响因子：
3.7
作者：
Lock FE;Hotchin NA
通讯作者：
Hotchin NA
影响因子：
15.9
作者：
Chapman, Sandra;Liu, Xuefeng;McBride, Alison A.
通讯作者：
McBride, Alison A.