Cryopreservation of sperm of Xenopus laevis and Xenopus tropicalis
Cryopreservation of sperm of Xenopus laevis and Xenopus tropicalis
复制标题
非洲爪蟾和热带爪蟾精子的超低温保存
作者：
M. Sargent;T. Mohun
现在，非洲爪蟾和X。tropicalis的基因组序列可以有效地产生，并与X.热带两栖类的早期发育可以用综合的生物化学和遗传学方法进行研究。然而，圈养大量在遗传筛选期间产生的动物或作为新的转基因品系产生的动物提出了相当大的挑战。我们描述了一种冷冻保存非洲爪蟾精子的方法，该方法应有助于低维护，长期储存雄性配子。通过优化冷冻保护剂、冷却和解冻速率以及受精条件，精子数量相当于X的八分之一。leevis testis或两个X.热带种的睾丸已经被冷冻保存，并在解冻后用于使这两个物种的卵受精。精子在冻融循环期间经历大量的活力丧失，但足以存活以使卵子受精。诱变青蛙的配子被储存起来，用于筛选发育突变。创世纪41：41-46，2005。© 2005 Wiley利斯公司
Now that transgenic strains of Xenopus laevis and X. tropicalis can be generated efficiently and with genomic sequence resources available for X. tropicalis, early amphibian development can be studied using integrated biochemical and genetic approaches. However, housing large numbers of animals generated during genetic screens or produced as novel transgenic lines presents a considerable challenge. We describe a method for cryopreserving Xenopus sperm that should facilitate low maintenance, long‐term storage of male gametes. By optimising the cryoprotectant, the rates of cooling and thawing, and conditions for fertilisation, sperm from the equivalent of one‐eighth of a X. laevis testis or of two X. tropicalis testes have been cryopreserved and used to fertilise eggs of both species after thawing. Sperm undergo a substantial loss of viability during a freeze‐thaw cycle, but sufficient survive to fertilise eggs. Gametes of mutagenised frogs are being stored in connection with a screen for developmental mutations. genesis 41:41–46, 2005. © 2005 Wiley‐Liss, Inc.
影响因子：
3.6
作者：
Visconti, PE;Stewart-Savage, J;Tezón, JG
通讯作者：
Tezón, JG
影响因子：
2.5
作者：
Doyong Gao;J. Critser
通讯作者：
Doyong Gao;J. Critser