Chloroplastic SaNADP-ME4 of C(3)-C(4) Woody Desert Species Salsola laricifolia Confers Drought and Salt Stress Resistance to Arabidopsis.
Chloroplastic SaNADP-ME4 of C(3)-C(4) Woody Desert Species Salsola laricifolia Confers Drought and Salt Stress Resistance to Arabidopsis.
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C（3）-C（4）木本荒漠植物松叶猪毛菜叶绿体SaNADP-ME 4对拟南芥抗旱耐盐性的影响
DOI：
10.3390/plants10091827
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发表时间：
2021-09-03
期刊：
影响因子：
--
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Wen Z;Wang Y;Xia C;Zhang Y;Zhang H
NADP-苹果酸酶 (NADP-ME) 在二价阳离子存在下催化 L-苹果酸的可逆脱羧反应产生丙酮酸、CO2 和 NADPH。此外，这种酶在植物发育和环境反应中发挥着至关重要的作用，特别是对于质体异构体。然而，与 C3 和 C4 物种相比，在干旱和盐胁迫下 C3-C4 中间物种中这种亚型的研究较少。在本研究中，我们从中间木本沙漠物种落叶猪毛菜中表征了 SaNADP-ME4。 SaNADP-ME4 编码 646 个氨基酸的蛋白质，根据共聚焦成像发现该蛋白质位于叶绿体中。实时定量PCR分析表明，SaNADP-ME4在叶中高表达，其次是茎和根，且SaNADP-ME4表达量分别在200 mm甘露醇和100 mm NaCl处理下得到提高并达到最大。过表达SaNADP-ME4的拟南芥在幼苗阶段的甘露醇和盐胁迫条件下表现出根长和鲜重增加。在成虫阶段，SaNADP-ME4可以缓解叶绿素含量和PSII光化学效率的下降，并提高超氧化物歧化酶、过氧化物酶和吡咯啉-5-羧酸合酶基因的表达，增强活性氧清除能力和脯氨酸水平。我们的结果表明，拟南芥中 SaNADP-ME4 的过表达可增强干旱和盐胁迫的抗性。
The NADP-malic enzyme (NADP-ME) catalyzes the reversible decarboxylation of L-malate to produce pyruvate, CO2, and NADPH in the presence of a bivalent cation. In addition, this enzyme plays crucial roles in plant developmental and environment responses, especially for the plastidic isoform. However, this isoform is less studied in C3–C4 intermediate species under drought and salt stresses than in C3 and C4 species. In the present study, we characterized SaNADP-ME4 from the intermediate woody desert species Salsola laricifolia. SaNADP-ME4 encoded a protein of 646 amino acids, which was found to be located in the chloroplasts based on confocal imaging. Quantitative real-time PCR analysis showed that SaNADP-ME4 was highly expressed in leaves, followed by stems and roots, and SaNADP-ME4 expression was improved and reached its maximum under the 200 mm mannitol and 100 mm NaCl treatments, respectively. Arabidopsis overexpressing SaNADP-ME4 showed increased root length and fresh weight under mannitol and salt stress conditions at the seedling stage. In the adult stage, SaNADP-ME4 could alleviate the decreased in chlorophyll contents and PSII photochemical efficiency, as well as improve the expression of superoxide dismutase, peroxidase, and pyrroline-5-carboxylate synthase genes to enhance reactive oxygen species scavenging capability and proline levels. Our results suggest that SaNADP-ME4 overexpression in Arabidopsis increases drought and salt stress resistance.
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影响因子：
56.9
作者：
BOYER, JS
通讯作者：
BOYER, JS
影响因子：
4.3
作者：
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5.2
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