Induction of heme oxygenase-1 in normal and malignant B lymphocytes by 15-deoxy-Delta(12,14)-prostaglandin J(2) requires Nrf2.
Induction of heme oxygenase-1 in normal and malignant B lymphocytes by 15-deoxy-Delta(12,14)-prostaglandin J(2) requires Nrf2.
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DOI：
10.1016/j.cellimm.2009.12.003
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发表时间：
2010
影响因子：
4.3
通讯作者：
Phipps, Richard P.
中科院分区：
文献类型：
--
作者：
Bancos, Simona;Baglole, Carolyn J.;Rahman, Irfan;Phipps, Richard P.
血红素加氧酶-1 （HO-1）是氧化应激诱导的，被认为是一种细胞保护和抗炎酶。目前尚不清楚正常或恶性的人B系细胞是否表达HO-1。15-deoxy-Δ12, 14-前列腺素J2 （15d-PGJ2）是一种有趣的亲电脂质介质，能够增加B细胞的氧化应激。在这里，我们测试了正常和恶性人B系细胞响应15d-PGJ2表达HO-1的能力，以及HO-1表达所需的信号通路。15d-PGJ2可诱导正常和恶性B细胞HO-1蛋白表达。与正常B淋巴细胞相比，恶性B细胞表现出更大的HO-1蛋白诱导。利用siRNA靶向转录因子Nrf2和从Nrf2缺失小鼠中分离的B细胞，我们发现15d-PGJ2诱导HO-1依赖于Nrf2。这些结果表明，与正常B淋巴细胞相比，恶性B细胞在响应15d-PGJ2时具有更大的增加HO-1蛋白水平的能力。我们推测，恶性B细胞高度表达HO-1的能力可能赋予生存优势。
Heme-oxygenase-1 (HO-1) is induced in response to oxidative stress and is believed to be a cytoprotective and anti-inflammatory enzyme. It is unknown whether normal or malignant human B lineage cells express HO-1. 15-deoxy-Δ12, 14-prostaglandin J2 (15d-PGJ2) is an interesting electrophilic lipid mediator able to increase oxidative stress in B cells. Here, we tested normal and malignant human B lineage cells for their ability to express HO-1 in response to 15d-PGJ2, as well as the signaling pathways required for HO-1 expression. 15d-PGJ2 potently induced HO-1 protein expression in normal and malignant B cells. Malignant B cells exhibited a greater induction of HO-1 protein compared to normal B lymphocytes. Using siRNA directed against the transcription factor Nrf2 and B cells isolated from Nrf2-deficient mice, we show that HO-1 induction by 15d-PGJ2 is dependent on Nrf2. These results show that, compared to normal B lymphocytes, malignant B cells have a greater capacity to increase their HO-1 protein levels in response to 15d-PGJ2. We speculate that the ability to highly express HO-1 by malignant B cells could confer a survival advantage.
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影响因子：
5.3
作者：
Kimpara, T;Takeda, A;Shibahara, S
通讯作者：
Shibahara, S
影响因子：
3.5
作者：
Hong, Hye-Young;Jeon, Woo-Kwang;Kim, Byung-Chul
通讯作者：
Kim, Byung-Chul
DOI：
10.1152/ajplung.90215.2008
发表时间：
2008-10-01
影响因子：
4.9
作者：
Baglole, Carolyn J.;Sime, Patricia J.;Phipps, Richard P.
通讯作者：
Phipps, Richard P.
影响因子：
5.8
作者：
Kim, Ji-Woo;Li, Mei-Hua;Surh, Young-Joon
通讯作者：
Surh, Young-Joon
DOI：
10.1006/bbrc.1997.6943
发表时间：
1997-07-18
影响因子：
3.1
作者：
Itoh, K;Chiba, T;Nabeshima, Y
通讯作者：
Nabeshima, Y