Non-invasive three-dimensional imaging of Escherichia coli K1 infection using diffuse light imaging tomography combined with micro-computed tomography.
Non-invasive three-dimensional imaging of Escherichia coli K1 infection using diffuse light imaging tomography combined with micro-computed tomography.
复制标题
使用漫射光成像断层扫描结合微型计算机断层扫描对大肠杆菌 K1 感染进行无创三维成像。
DOI：
10.1016/j.ymeth.2017.05.005
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Witcomb LA
中科院分区：
文献类型：
--
作者：
Witcomb LA
与二维生物发光成像相比，三维漫射光成像断层扫描与集成微计算机断层扫描（DLIT-μCT）有可能实现感染模式的空间变化时，成像实验动物与携带生物发光报告基因的毒性细菌的衍生物，如来自细菌Photorhabdus luminescens的theluxoperon。该方法提供了精确定位动物体内细菌感染部位的机会，并能够生成感染周期的四维电影。在这里，我们描述了使用PerkinElmer IVIS SpectrumCT体内成像系统，以调查新生大鼠胃肠道定植新生儿病原体大肠杆菌K1后致死性全身感染的进展。我们证实了先前的观察结果，即这些细菌在从微量移液器喂食感染剂量后稳定地定殖在结肠和小肠中;入侵的细菌穿过肠道上皮迁移到血液循环中，并在主要器官（包括大脑）中建立感染灶。DLIT-μCT显示了消化道内新的多个定植部位，包括舌、食管和胃，穿透了非角化食管上皮表面，为细菌传播的另一个主要部位提供了有力证据。我们强调了与新生大鼠幼仔感染成像相关的技术问题，并表明全身和器官生物负载与疾病严重程度相关。
In contrast to two-dimensional bioluminescence imaging, three dimensional diffuse light imaging tomography with integrated micro-computed tomography (DLIT-μCT) has the potential to realise spatial variations in infection patterns when imaging experimental animals dosed with derivatives of virulent bacteria carrying bioluminescent reporter genes such as theluxoperon from the bacteriumPhotorhabdus luminescens. The method provides an opportunity to precisely localise the bacterial infection sites within the animal and enables the generation of four-dimensional movies of the infection cycle. Here, we describe the use of the PerkinElmer IVIS SpectrumCTin vivoimaging system to investigate progression of lethal systemic infection in neonatal rats following colonisation of the gastrointestinal tract with the neonatal pathogenEscherichia coliK1. We confirm previous observations that these bacteria stably colonize the colon and small intestine following feeding of the infectious dose from a micropipette; invading bacteria migrate across the gut epithelium into the blood circulation and establish foci of infection in major organs, including the brain. DLIT-μCT revealed novel multiple sites of colonisation within the alimentary canal, including the tongue, oesophagus and stomach, with penetration of the non-keratinised oesophageal epithelial surface, providing strong evidence of a further major site for bacterial dissemination. We highlight technical issues associated with imaging of infections in new born rat pups and show that the whole-body and organ bioburden correlates with disease severity.
影响因子：
9.8
作者：
Chemali, J. J.;Kenny, J. D.;Solt, K.
通讯作者：
Solt, K.
大肠杆菌侵袭力与K1荚膜多糖的关系综述
影响因子：
3.6
作者：
M. Schiffer;Elizabeth Oliveira;M. Glodé;G. Mccracken;Larry M Sarff;J. Robbins
通讯作者：
J. Robbins
影响因子：
3.8
作者：
Cong, WX;Wang, G;Cong, A
通讯作者：
Cong, A