A novel mini-DNA barcoding assay to identify processed fins from internationally protected shark species.
A novel mini-DNA barcoding assay to identify processed fins from internationally protected shark species.
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DOI：
10.1371/journal.pone.0114844
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Chapman DD
中科院分区：
文献类型：
--
作者：
Fields AT;Abercrombie DL;Eng R;Feldheim K;Chapman DD
越来越需要确定贸易中的鲨鱼产品，部分原因是最近将五种具有重要商业价值的物种列入《濒危物种国际贸易公约》(CITES；除了本世纪初列出的三个物种（鲸类，Rhincodon typus, basking， Cetorhinus maximus和白鲸类，Carcharodon carcharias）之外，还有鼠头鲸，蓝鳍鲸，海洋白鳍鲨，长形双髻鲨，lewini，光滑双髻鲨，S. zygaena和大双髻鲨。鱼翅在国际上进行交易，供应给亚洲干海鲜市场，在亚洲，鱼翅被用来制作鱼翅汤。鱼翅进入国际贸易时通常皮肤完好无损，可以通过形态学特征或标准dna条形码方法进行识别。一旦它们到达亚洲并在该地区进行交易，它们的皮肤就会被去除，并经过化学处理，消除许多关键的诊断特征，并降解它们的DNA（“加工鳍”）。在这里，我们提出了一种基于细胞色素氧化酶I基因部分序列的有效迷你条形码检测方法，可以可靠地识别出8种CITES列出的鲨鱼物种中的7种的加工过的鳍。我们还证明，该检测甚至可以频繁地识别鱼翅汤的种类或属（50个样本中的31个）。
There is a growing need to identify shark products in trade, in part due to the recent listing of five commercially important species on the Appendices of the Convention on International Trade in Endangered Species (CITES; porbeagle, Lamna nasus, oceanic whitetip, Carcharhinus longimanus scalloped hammerhead, Sphyrna lewini, smooth hammerhead, S. zygaena and great hammerhead S. mokarran) in addition to three species listed in the early part of this century (whale, Rhincodon typus, basking, Cetorhinus maximus, and white, Carcharodon carcharias). Shark fins are traded internationally to supply the Asian dried seafood market, in which they are used to make the luxury dish shark fin soup. Shark fins usually enter international trade with their skin still intact and can be identified using morphological characters or standard DNA-barcoding approaches. Once they reach Asia and are traded in this region the skin is removed and they are treated with chemicals that eliminate many key diagnostic characters and degrade their DNA (“processed fins”). Here, we present a validated mini-barcode assay based on partial sequences of the cytochrome oxidase I gene that can reliably identify the processed fins of seven of the eight CITES listed shark species. We also demonstrate that the assay can even frequently identify the species or genus of origin of shark fin soup (31 out of 50 samples).
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影响因子：
3.8
作者：
Worm, Boris;Davis, Brendal;Gruber, Samuel H.
通讯作者：
Gruber, Samuel H.
DOI：
10.1098/rstb.2005.1716
发表时间：
2005-10-29
影响因子：
6.3
作者：
Ward, RD;Zemlak, TS;Hebert, PDN
通讯作者：
Hebert, PDN
影响因子：
6.3
作者：
Clarke, SC;Magnussen, JE;Shivji, MS
通讯作者：
Shivji, MS
影响因子：
2.2
作者：
Dawnay, Nick;Ogden, Rob;Thorpe, Roger S.
通讯作者：
Thorpe, Roger S.
影响因子：
7.7
作者：
Wong, Eugene H. -K.;Shivji, Mahmood S.;Hanner, Robert H.
通讯作者：
Hanner, Robert H.