Endothelial Cellular Responses to Biodegradable Metal Zinc.
Endothelial Cellular Responses to Biodegradable Metal Zinc.
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DOI：
10.1021/acsbiomaterials.5b00319
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发表时间：
2015
影响因子：
5.8
通讯作者：
Zhu, Donghui
中科院分区：
文献类型：
--
作者：
Ma, Jun;Zhao, Nan;Zhu, Donghui
可生物降解锌金属材料是新一代生物材料，具有良好的生物可降解性、生物可吸收性和组织再生适应性。与镁（Mg）和铁（Fe）相比，锌在骨科和支架应用中具有更好的腐蚀和机械性能。植入后，含锌材料会缓慢降解，锌离子（Zn2+）会释放到周围组织中。对于支架应用，内皮组织/细胞附近的局部Zn 2+浓度可能很高。然而，目前尚不清楚内皮细胞将如何响应如此高浓度的Zn 2+，这对血管重塑和再生至关重要。在这里，我们评估了暴露于浓度梯度（0 - 140 μ M）的细胞外Zn2+的原代人冠状动脉内皮细胞（HCEC）的短期细胞行为。Zn2+对细胞活力、增殖、扩散和迁移具有有趣的双相效应。一般来说，低浓度的Zn 2+促进活力，增殖，粘附和迁移，而高浓度的Zn 2+有相反的效果。对于基因表达谱，受影响最大的功能基因与细胞粘附、细胞损伤、细胞生长、血管生成、炎症、血管张力和凝血有关。这些结果为锌基合金的设计以及支架中锌离子的控制释放和其他相关的医疗应用提供了有益的信息和指导。
Biodegradable zinc (Zn) metals, a new generation of biomaterials, have attracted much attention due to their excellent biodegradability, bioabsorbability, and adaptability to tissue regeneration. Compared with magnesium (Mg) and iron (Fe), Zn exhibits better corrosion and mechanical behaviors in orthopedic and stent applications. After implantation, Zn containing material will slowly degrade, and Zn ions (Zn2+) will be released to the surrounding tissue. For stent applications, the local Zn2+concentration near endothelial tissue/cells could be high. However, it is unclear how endothelia will respond to such high concentrations of Zn2+, which is pivotal to vascular remodeling and regeneration. Here, we evaluated the short-term cellular behaviors of primary human coronary artery endothelial cells (HCECs) exposed to a concentration gradient (0−140 μM) of extracellular Zn2+. Zn2+ had an interesting biphasic effect on cell viability, proliferation, spreading, and migration. Generally, low concentrations of Zn2+ promoted viability, proliferation, adhesion, and migration, while high concentrations of Zn2+ had opposite effects. For gene expression profiles, the most affected functional genes were related to cell adhesion, cell injury, cell growth, angiogenesis, inflammation, vessel tone, and coagulation. These results provide helpful information and guidance for Zn-based alloy design as well as the controlled release of Zn2+in stent and other related medical applications.
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影响因子：
2.9
作者：
Bosiers, Marc
通讯作者：
Bosiers, Marc
影响因子：
14
作者：
Lipski, Anna M.;Pino, Christopher J.;Haselton, Frederick R.;Chen, I-Wei;Shastri, V. Prasad
通讯作者：
Shastri, V. Prasad
影响因子：
15.9
作者：
MARUI, N;OFFERMANN, MK;MEDFORD, RM
通讯作者：
MEDFORD, RM
影响因子：
4.8
作者：
McMullen, ME;Bryant, PW;Pumiglia, KM
通讯作者：
Pumiglia, KM
DOI：
10.1016/0167-4781(94)00237-w
发表时间：
1995-03-14
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
MARTINOTTI, S;TONIATO, E;GULINO, A
通讯作者：
GULINO, A