The effect of silica nanoparticle-modified surfaces on cell morphology, cytoskeletal organization and function.
The effect of silica nanoparticle-modified surfaces on cell morphology, cytoskeletal organization and function.
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DOI：
10.1016/j.biomaterials.2008.06.002
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发表时间：
2008-10
期刊：
影响因子：
14
通讯作者：
Shastri, V. Prasad
中科院分区：
文献类型：
--
作者：
Lipski, Anna M.;Pino, Christopher J.;Haselton, Frederick R.;Chen, I-Wei;Shastri, V. Prasad
关键词：
生物材料表面的化学和形态特征被认为在决定细胞分化和凋亡中起重要作用。在这份报告中，我们研究了纳米颗粒（NP）组件排列在一个平面基板上的细胞骨架组织，增殖和代谢活性的两种细胞类型，牛主动脉内皮细胞（BAECs）和小鼠颅骨前成骨细胞（MC 3 T3-E1）的影响。为了在不改变化学性质的情况下改变粗糙度，用直径为50、100和300 nm的单分散二氧化硅纳米颗粒涂覆玻璃基材。在纳米级的表面粗糙度对细胞形态的影响进行了研究，通过量化细胞的扩散，形状，细胞骨架F-肌动蛋白的对齐，并使用图像分析的粘着斑复合物（FAC）的招聘。使用噻唑蓝溴化四唑测定跟踪代谢活性。在测试的两种细胞类型中，纳米颗粒引入的表面粗糙度对细胞形态和代谢具有细胞类型特异性影响。虽然与在玻璃上生长的BAEC相比，NP改性基底上的BAEC表现出较小的细胞面积和较少的粘着斑复合物，但与未改性玻璃相比，MC 3 T3-E1细胞在NP改性表面上表现出较大的细胞面积和增加的FACs数量。然而，在50 nm NP上的两种细胞类型具有最高的增殖率（与玻璃对照相当），而在300 nm NP上生长的细胞表现出增殖抑制。有趣的是，对于这两种细胞类型，表面粗糙度促进了长而粗的F-肌动蛋白纤维的形成，这些纤维与每个细胞的长轴对齐。这些发现与我们早期的研究结果一致，即人间充质祖细胞的成骨分化在NP修饰的表面上增强。我们的发现，纳米粗糙度，赋予纳米粒子组件，影响细胞的过程中的细胞特异性的方式，可以有深远的影响的发展“智能”生物材料，特别是指导干细胞分化。
Chemical and morphological characteristics of a biomaterial surface are thought to play an important role in determining cellular differentiation and apoptosis. In this report, we investigate the effect of nanoparticle (NP) assemblies arranged on a flat substrate on cytoskeletal organization, proliferation and metabolic activity on two cell types, Bovine aortic endothelial cells (BAECs) and mouse calvarial preosteoblasts (MC3T3-E1). To vary roughness without altering chemistry, glass substrates were coated with monodispersed silica nanoparticles of 50, 100 and 300 nm in diameter. The impact of surface roughness at the nanoscale on cell morphology was studied by quantifying cell spreading, shape, cytoskeletal F-actin alignment, and recruitment of focal adhesion complexes (FAC) using image analysis. Metabolic activity was followed using a thiazolyl blue tetrazolium bromide assay. In the two cell types tested, surface roughness introduced by nanoparticles had cell type specific effects on cell morphology and metabolism. While BAEC on NP-modified substrates exhibited smaller cell areas and fewer focal adhesion complexes compared to BAEC grown on glass, MC3T3-E1 cells in contrast exhibited larger cell areas on NP-modified surfaces and an increased number of FACs, in comparison to unmodified glass. However, both cell types on 50 nm NP had the highest proliferation rates (comparable to glass control) whereas cells grown on 300 nm NP exhibited inhibited proliferation. Interestingly, for both cell types surface roughness promoted the formation of long, thick F-actin fibers, which aligned with the long axis of each cell. These findings are consistent with our earlier result that osteogenic differentiation of human mesenchymal progenitor cells is enhanced on NP-modified surfaces. Our finding that nanoroughness, as imparted by nanoparticle assemblies, effects cellular processes in a cell specific manner, can have far reaching consequences on the development of “smart” biomaterials especially for directing stem cell differentiation.
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DOI：
10.1002/jbm.10273
发表时间：
2002-12-05
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Itälä, A;Ylänen, HO;Aro, HT
通讯作者：
Aro, HT
影响因子：
29.4
作者：
Lipski, Anna Marie;Jaquiery, Claude;Shastri, Venkatram Prasad
通讯作者：
Shastri, Venkatram Prasad
影响因子：
7.5
作者：
Boudreau, N;Bissell, MJ
通讯作者：
Bissell, MJ
影响因子：
14
作者：
Anselme, K;Linez, P;Leroy, JM
通讯作者：
Leroy, JM
影响因子：
3.9
作者：
Curtis, ASG;Gadegaard, N;Aitchison, G
通讯作者：
Aitchison, G