Validation of a radioimmunoassay for measuring fecal cortisol metabolites in the hystricomorph rodent, Octodon degus.
Validation of a radioimmunoassay for measuring fecal cortisol metabolites in the hystricomorph rodent, Octodon degus.
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验证用于测量八齿八齿鼠粪便皮质醇代谢物的放射免疫测定法。
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
L. Ebensperger
中科院分区：
文献类型：
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作者：
M. Soto;Sandra P. Gonzalez;L. Hayes;L. Ebensperger
粪便类固醇代谢物的测定是一种非侵入性技术，其表征生物体的生理状态，而没有处理的生理和心理压力。虽然这种技术在野生动物和/或圈养动物的研究中有许多应用，而不需要捕获个体，但在使用前需要进行物种特异性验证。完整的验证包括分析和生理验证。在后者中，粪便激素代谢物的变化由先前对相应血浆激素的操作诱导。在这里，我们验证了测量粪便皮质醇代谢产物（FCM）的方法在hystricomorph啮齿类动物degus。我们用80%乙醇提取粪便，并使用市售皮质醇放射免疫测定法定量类固醇。我们首先比较了基线水平的血皮质醇和FCM，然后进行了挑战试验与促肾上腺皮质激素（ACTH），以证明FCM准确地反映肾上腺皮质活动。我们发现血皮质醇浓度与其粪便代谢物浓度之间存在显著正相关。在促肾上腺皮质激素的挑战试验中，血液皮质醇水平达到峰值后30分钟注射，FCM反映了这个峰值的延迟约6 hr. Our成功验证的非侵入性的方法提供了新的机会，研究评估自然条件下的社会和生态因素对degus的影响。
Determination of fecal steroid metabolites is a noninvasive technique that characterizes the physiological state of organisms without the physiological and psychological stress of handling. Although this technique has many applications in the study of wildlife and/or captive animals without the necessity of capturing individuals, it requires a species-specific validation before use. A complete validation includes an analytical and a physiological one. In the latter changes in fecal hormone metabolites are induced by previous manipulations of the respective plasma hormones. Here we validated a method for measuring fecal cortisol metabolites (FCM) in the hystricomorph rodent Octodon degus. We extracted feces with 80% ethanol and quantified steroids using a commercial available cortisol radioimmunoassay. We first compared baseline levels of blood cortisol and FCM, and then performed a challenge test with adrenocorticotropic hormone (ACTH) to demonstrate that FCM accurately reflect adrenocortical activity. We found a significantly positive relationship between concentrations of blood cortisol and its fecal metabolites. During the ACTH challenge test, blood cortisol levels peaked 30 min after injection, and FCM mirrored this peak with a delay of about 6 hr. Our successfully validated noninvasive method provides new opportunities for studies assessing the influence of social and ecological factors on degus under natural conditions.
影响因子：
2.7
作者：
Wasser, SK;Hunt, KE;Monfort, SL
通讯作者：
Monfort, SL