Regional and cellular distribution of the P2Y1 purinergic receptor in the human brain: Striking neuronal localisation
Regional and cellular distribution of the P2Y1 purinergic receptor in the human brain: Striking neuronal localisation
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人脑中 P2Y1 嘌呤能受体的区域和细胞分布：引人注目的神经元定位
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
P. Emson
中科院分区：
文献类型：
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作者：
Darren Moore;J. Chambers;H. Waldvogel;R. Faull;P. Emson
细胞外核苷酸的生物学作用通过P2受体P2X和P2Y两个家族发挥作用。代谢性P2Y受体包括至少7种不同的亚型，这些亚型已经从许多物种中克隆出来。然而，目前还没有一种P2Y受体蛋白在人脑中被观察到。本研究采用免疫组织化学方法研究了P2Y1受体在人脑中的区域分布和细胞分布。针对P2Y1蛋白C端合成的肽段，制备了多克隆抗体。免疫印迹分析表明，P2Y1抗血清特异性识别人和大鼠脑膜中的63 - kDa条带。同样，抗血清在转染的1321N1细胞中特异性检测到人P2Y1受体。免疫组织化学分析灌注固定人脑组织显示该受体在整个大脑中广泛分布。在细胞水平上，P2Y1受体明显定位于大脑皮层、小脑皮层、海马、尾状核、壳核、苍白球、丘脑底核、红核和中脑的神经元结构。在其他非神经元细胞类型中未检测到P2Y1受体的表达。这些结果提供了P2Y受体在人脑中的细胞分布的第一个特征。P2Y1受体的广泛和丰富的分布表明它参与了人类大脑的许多重要功能。这种受体的神经元定位指出了在神经传递中可能起的作用，也强调了细胞外核苷酸作为大脑内信号分子的主要作用。[j] .中华神经科杂志，2004,21(2):344 - 344。©2000 Wiley‐Liss, Inc。
The biological actions of extracellular nucleotides are exerted via two families of P2 receptors, P2X and P2Y. The metabotropic P2Y receptors comprise at least 7 distinct subtypes, which have been cloned from a number of species. However, none of the P2Y receptor proteins have been visualised yet in human brain. In the present study, the regional and cellular distribution of the P2Y1 receptor was investigated in the human brain by using immunohistochemistry. Polyclonal antibodies were raised against a synthetic peptide from the C‐terminus of the P2Y1 protein. Immunoblot analysis demonstrated that P2Y1 antiserum specifically recognised a 63‐kDa band in human and rat brain membranes. Similarly, the antiserum specifically detected the human P2Y1 receptor in transfected 1321N1 cells. Immunohistochemical analysis on perfusion‐fixed human brain tissue showed a widespread distribution for this receptor throughout the brain. At the cellular level, the P2Y1 receptor was strikingly localised to neuronal structures of the cerebral cortex, cerebellar cortex, hippocampus, caudate nucleus, putamen, globus pallidus, subthalamic nucleus, red nucleus, and midbrain. Expression of the P2Y1 receptor was not detected in other non‐neuronal cell types. These results provide the first characterisation of the cellular distribution of a P2Y receptor in the human brain. The widespread and abundant distribution of the P2Y1 receptor suggests its involvement in a number of important functions within the human brain. The neuronal localisation of this receptor points towards a possible role in neurotransmission, and also highlights a major role for extracellular nucleotides as signaling molecules within the brain. J. Comp. Neurol. 421:374–384, 2000. © 2000 Wiley‐Liss, Inc.
大鼠和小鼠 P2Y 嘌呤受体的克隆。
DOI：
10.1006/bbrc.1995.1798
发表时间：
1995
期刊：
Biochemical and biophysical research communications.
影响因子：
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作者：
Tokuyama,Y;Hara,M;Jones,EM;Fan,Z;Bell,GI
通讯作者：
Bell,GI
与磷脂酶 C 偶联的克隆 P2Y 嘌呤能受体的表达。
影响因子：
3.6
作者：
Filtz,TM;Li,Q;Boyer,JL;Nicholas,RA;Harden,TK
通讯作者：
Harden,TK
三磷酸腺苷对人类 P2Y1 受体的激动作用。
影响因子：
3.6
作者：
Palmer,RK;Boyer,JL;Schachter,JB;Nicholas,RA;Harden,TK
通讯作者：
Harden,TK