The pre-activated immune response induced by LPS protects host from leptospirosis.
The pre-activated immune response induced by LPS protects host from leptospirosis.
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LPS 诱导的预激活免疫反应可保护宿主免受钩端螺旋体病的侵害
DOI：
10.1371/journal.pone.0242742
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Cao Y
中科院分区：
文献类型：
--
作者：
Chen X;Xie X;Wu D;Zhang S;Zhang W;Cao Y
钩端螺旋体病是由致病性钩端螺旋体引起的一种重要的全球性人畜共患病。据估计，每年有超过100万人感染钩端螺旋体，死亡人数约为6万人。研究表明，延迟的免疫应答与钩端螺旋体病的严重程度有关，TLR 4在钩端螺旋体病的防治中起着重要作用。在这项研究中，我们的目的是探讨经典的激活剂（LPS）的TLR 4在钩体病的敏感和耐药主机的影响。结果表明，LPS预处理可使仓鼠的存活率提高到80%。LPS预处理还能显著降低钩体负荷，减轻仓鼠和小鼠脏器的病理损伤。ELISA检测结果显示，LPS治疗组小鼠血清TNF-α和IL-1β水平较感染前对照组升高。感染后2d，LPS组细胞因子水平较对照组明显下降。此外，体外实验结果表明，LPS预处理增强了巨噬细胞对钩端螺旋体的吞噬和杀菌能力。以上结果表明，LPS诱导的预激活免疫应答增强了宿主对钩体病的抵抗能力。
Leptospirosis is an important global zoonosis caused by pathogenic Leptospira. It is estimated that more than 1 million people are infected by Leptospira each year, and the death toll is about 60,000. Some studies showed that delayed immune response was associated with severe leptospirosis, and TLR4 was very important in the control of leptospirosis. In this study, we aimed to explore the effect of the classical activator (LPS) of TLR4 on leptospirosis in susceptible and resistant hosts. The results showed that LPS pretreatment increased the survival rate of hamsters to 80%. And LPS pre-treatment also significantly reduced the leptospiral load and alleviated the pathological injury in organs of hamsters and mice. The result detected by ELISA in mice showed that the levels of TNF-α and IL-1β were increased in the LPS-treated group compared to the control group before infection. However, two days after infection, the level of cytokines in LPS group was down-regulated compared with that in control group. In addition, in vitro results showed that LPS pre-treatment enhanced the phagocytosis and bactericidal ability of macrophages on Leptospira. Collectively, our results indicated that the pre-activated immune response induced by LPS enhanced the ability of host against leptospirosis.
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DOI：
10.1093/trstmh/trt112
发表时间：
2014-02-01
影响因子：
2.2
作者：
Soares, Luciane Marieta;Macedo, Julio Oliveira;Athanazio, Daniel Abensur
通讯作者：
Athanazio, Daniel Abensur
钩端螺旋体病理生理学：进入细胞因子的风暴。
影响因子：
5.7
作者：
Cagliero J;Villanueva SYAM;Matsui M
通讯作者：
Matsui M
影响因子：
5.5
作者：
BARTH, MW;HENDRZAK, JA;MORAHAN, PS
通讯作者：
MORAHAN, PS
影响因子：
3.7
作者：
Guerrier G;D'Ortenzio E
通讯作者：
D'Ortenzio E
在钩端螺旋体病期间，单核巨噬细胞而非中性粒细胞充当主要的浸润性抗钩端螺旋体吞噬细胞
影响因子：
3.7
作者：
Chen X;Li SJ;Ojcius DM;Sun AH;Hu WL;Lin X;Yan J
通讯作者：
Yan J