Development of a chemically defined medium and discovery of new mitogenic growth factors for mouse hepatocytes: mitogenic effects of FGF1/2 and PDGF.
Development of a chemically defined medium and discovery of new mitogenic growth factors for mouse hepatocytes: mitogenic effects of FGF1/2 and PDGF.
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DOI：
10.1371/journal.pone.0095487
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Michalopoulos GK
中科院分区：
文献类型：
--
作者：
Bowen WC;Michalopoulos AW;Orr A;Ding MQ;Stolz DB;Michalopoulos GK
Chemically defined serum-free media for rat hepatocytes have been useful in identifying EGFR ligands and HGF/MET signaling as direct mitogenic factors for rat hepatocytes. The absence of such media for mouse hepatocytes has prevented screening for discovery of such mitogens for mouse hepatocytes. We present results obtained by designing such a chemically defined medium for mouse hepatocytes and demonstrate that in addition to EGFR ligands and HGF, the growth factors FGF1 and FGF2 are also important mitogenic factors for mouse hepatocytes. Smaller mitogenic response was also noticed for PDGF AB. Mouse hepatocytes are more likely to enter into spontaneous proliferation in primary culture due to activation of cell cycle pathways resulting from collagenase perfusion. These results demonstrate unanticipated fundamental differences in growth biology of hepatocytes between the two rodent species.
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影响因子：
5.8
作者：
Michalopoulos, George K.
通讯作者：
Michalopoulos, George K.
DOI：
10.1073/pnas.0409722102
发表时间：
2005-03-01
影响因子：
11.1
作者：
Campbell, JS;Hughes, SD;Fausto, N
通讯作者：
Fausto, N
影响因子：
6
作者：
Yu, CD;Wang, F;McKeehan, WL
通讯作者：
McKeehan, WL
影响因子：
4.8
作者：
Luo, Yongde;Yang, Chaofeng;McKeehan, Wallace L.
通讯作者：
McKeehan, Wallace L.
影响因子：
24.5
作者：
Uriarte, Iker;Fernandez-Barrena, Maite G.;Avila, Matias A.
通讯作者：
Avila, Matias A.