Enhancement of nitric oxide release from nitrosyl hemoglobin and nitrosyl myoglobin by red/near infrared radiation: potential role in cardioprotection.
Enhancement of nitric oxide release from nitrosyl hemoglobin and nitrosyl myoglobin by red/near infrared radiation: potential role in cardioprotection.
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DOI：
10.1016/j.yjmcc.2009.03.009
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发表时间：
2009-08
影响因子：
5
通讯作者：
Hogg, Neil
中科院分区：
文献类型：
--
作者：
Lohr, Nicole L.;Keszler, Agnes;Pratt, Phillip;Bienengraber, Martin;Warltier, David C.;Hogg, Neil
一氧化氮是许多生物学过程中的重要信使，如血管生成、缺氧血管舒张和心脏保护。虽然一氧化氮合酶（NOS）产生大量的NO，但在体内，特别是在低氧或缺氧期间，NOS独立产生NO越来越受到关注，其中低氧张力限制NOS活性。可以增加NO生物利用度的干预措施具有显著的治疗潜力。使用远红光和近红外光（R/NIR）可以减少梗死面积，保护神经元免受甲醇毒性，并刺激血管生成。R/NIR如何在体内和体外调节这些过程尚不清楚，但已表明涉及NO水平的增加。在这项研究中，我们研究了R/NIR光是否可以促进NO从亚硝基血红素蛋白的释放。此外，我们研究了R/NIR光是否可以增强亚硝酸盐对兔心脏缺血和再灌注损伤的保护作用。我们表明，在纯化的系统和心肌中，R/NIR光可以衰减亚硝酰血红素和释放NO，并且这种释放的NO可以增强亚硝酸盐的心脏保护作用。因此，光解离为NO及其与亚硝酸钠的协同效应可能代表了增加NO生物利用度的非侵入性和位点特异性手段。
Nitric oxide is an important messenger in numerous biological processes, such as angiogenesis, hypoxic vasodilation, and cardioprotection. Although nitric oxide synthases (NOS) produce the bulk of NO, there is increasing interest in NOS independent generation of NO in vivo, particularly during hypoxia or anoxia, where low oxygen tensions limit NOS activity. Interventions that can increase NO bioavailability have significant therapeutic potential. The use of far red and near infrared light (R/NIR) can reduce infarct size, protect neurons from methanol toxicity, and stimulate angiogenesis. How R/NIR modulates these processes in vivo and in vitro is unknown, but it has been suggested that increases in NO levels are involved. In this study we examined if R/NIR light could facilitate the release of NO from nitrosyl heme proteins. In addition, we examined if R/NIR light could enhance the protective effects of nitrite on ischemia and reperfusion injury in the rabbit heart. We show both in purified systems and in myocardium that R/NIR light can decay nitrosyl hemes and release NO, and that this released NO may enhance the cardioprotective effects of nitrite. Thus, the photodissociation to NO and its synergistic effect with sodium nitrite may represent a noninvasive and site specific means for increasing NO bioavailability.
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