Functional analysis of the α-1,3-glucan synthase genes agsA and agsB in Aspergillus nidulans: agsB is the major α-1,3-glucan synthase in this fungus.
Functional analysis of the α-1,3-glucan synthase genes agsA and agsB in Aspergillus nidulans: agsB is the major α-1,3-glucan synthase in this fungus.
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DOI：
10.1371/journal.pone.0054893
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Abe K
中科院分区：
文献类型：
--
作者：
Yoshimi A;Sano M;Inaba A;Kokubun Y;Fujioka T;Mizutani O;Hagiwara D;Fujikawa T;Nishimura M;Yano S;Kasahara S;Shimizu K;Yamaguchi M;Kawakami K;Abe K
虽然α-1，3-葡聚糖是丝状真菌中主要的细胞壁多糖之一，但α-1，3-葡聚糖的生理作用尚不清楚。构巢曲霉（Aspergillus nidulans）具有两个α-1，3-葡聚糖合成酶（AGS）基因agsA和agsB。为了对这些基因进行功能分析，我们构建了A. nidulans：agsA破坏、agsB破坏和双破坏菌株。我们还构建了几个CagsB菌株，其中agsB的表达由诱导型alcA启动子控制，有或没有agsA破坏突变。agsA破坏菌株没有显示出与野生型菌株明显不同的表型。agsB破坏菌株形成分散的菌丝细胞在液体培养条件下，无论agsA的遗传背景。分散的菌丝细胞也观察到CagsB菌株的液体培养时agsB的表达被抑制，而这些菌株生长正常，即使在平板培养agsB抑制条件下。基于其组分的碱溶解度、糖的定量和1,3 C-NMR光谱分析的细胞壁分级显示，α-1，3-葡聚糖是野生型和agsA破坏菌株中碱溶性级分的主要组分，但几乎没有α-1，3-葡聚糖，在agsB抑制条件下，在来源于agsB破坏菌株或CagsB菌株的碱溶性级分中发现β-葡聚糖，不管agsA基因背景如何。综上所述，我们的数据表明，这两个AGS基因是在A.但AgsB是液体培养条件下正常生长所必需的，是该物种中主要的AGS。
Although α-1,3-glucan is one of the major cell wall polysaccharides in filamentous fungi, the physiological roles of α-1,3-glucan remain unclear. The model fungus Aspergillus nidulans possesses two α-1,3-glucan synthase (AGS) genes, agsA and agsB. For functional analysis of these genes, we constructed several mutant strains in A. nidulans: agsA disruption, agsB disruption, and double-disruption strains. We also constructed several CagsB strains in which agsB expression was controlled by the inducible alcA promoter, with or without the agsA-disrupting mutation. The agsA disruption strains did not show markedly different phenotypes from those of the wild-type strain. The agsB disruption strains formed dispersed hyphal cells under liquid culture conditions, regardless of the agsA genetic background. Dispersed hyphal cells were also observed in liquid culture of the CagsB strains when agsB expression was repressed, whereas these strains grew normally in plate culture even under the agsB-repressed conditions. Fractionation of the cell wall based on the alkali solubility of its components, quantification of sugars, and 13C-NMR spectroscopic analysis revealed that α-1,3-glucan was the main component of the alkali-soluble fraction in the wild-type and agsA disruption strains, but almost no α-1,3-glucan was found in the alkali-soluble fraction derived from either the agsB disruption strain or the CagsB strain under the agsB-repressed conditions, regardless of the agsA genetic background. Taken together, our data demonstrate that the two AGS genes are dispensable in A. nidulans, but that AgsB is required for normal growth characteristics under liquid culture conditions and is the major AGS in this species.
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影响因子：
3.5
作者：
GWYNNE, DI;BUXTON, FP;SEALYLEWIS, HM
通讯作者：
SEALYLEWIS, HM
影响因子：
2.5
作者：
Hagiwara, Daisuke;Kondo, Atsushi;Abe, Keietsu
通讯作者：
Abe, Keietsu
DOI：
10.1073/pnas.95.16.9161
发表时间：
1998-08-04
影响因子：
11.1
作者：
Hochstenbach, F;Klis, FM;Klausner, RD
通讯作者：
Klausner, RD
影响因子：
1.6
作者：
Ichinomiya, Masayuki;Uchida, Hirotaka;Horiuchi, Hiroyuki
通讯作者：
Horiuchi, Hiroyuki
影响因子：
3
作者：
Fontaine, Thierry;Beauvais, Anne;Latge, Jean-Paul
通讯作者：
Latge, Jean-Paul