In Vitro Immunological Effects of CXCR3 Inhibitor AMG487 on Dendritic Cells
In Vitro Immunological Effects of CXCR3 Inhibitor AMG487 on Dendritic Cells
复制标题
CXCR3抑制剂AMG487对树突状细胞的体外免疫学影响
DOI：
10.1007/s00005-020-00577-3
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发表时间：
2020-04
影响因子：
3.2
通讯作者：
Ren Hanyun
中科院分区：
文献类型：
--
作者：
Qin Chenchen;Ren Hanyun
AMG 487是趋化因子受体CXCR 3的靶向阻断剂，通过阻断炎症周期来改善炎症症状。在此，我们研究了AMG 487是否影响树突状细胞（DC）的生物学和功能。DC上共刺激标志物的表达降低，表明在整个体外分化期间加入AMG 487时DC处于半成熟状态。此外，当仅在最终脂多糖诱导的活化步骤中添加时，AMG 487可抑制DC活化，如活化标志物表达减少所示。AMG 487还促进PD-L2的表达，并损害诱导抗原特异性T细胞应答的能力。我们的结果表明，AMG 487显著影响DC体外成熟和功能，导致T细胞活化受损，诱导DC具有与致耐受性DC相似的特征。AMG 487可能在DC发育和功能形成过程中直接发挥免疫调节作用。
AMG 487 is the targeted blocker of chemokine receptor CXCR3 and improves inflammatory symptoms by blocking the inflammatory cycle. Here we investigated whether AMG 487 affects dendritic cell (DC) biology and function. The expression of co-stimulatory markers on DCs was reduced, indicating the semi-mature state of DC when AMG 487 was added throughout the in vitro differentiation period. Additionally, when added solely during the final lipopolysaccharide-induced activation step, AMG 487 inhibited DC activation, as demonstrated by a decreased expression of activation markers. AMG487 also promoted the expression of PD-L2 and impaired the ability to induce antigen-specific T cell responses. Our results demonstrated that AMG 487 significantly affects DC maturity in vitro and function leading to impaired T cell activation, inducing DCs to have characteristics similar to tolerogenic DCs. AMG 487 may directly play an immunomodulatory role during DC development and functional shaping.
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DOI：
10.1084/jem.184.3.963
发表时间：
1996-09-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Loetscher M;Gerber B;Loetscher P;Jones SA;Piali L;Clark-Lewis I;Baggiolini M;Moser B
通讯作者：
Moser B
影响因子：
3.9
作者：
G. Tonn;Simon G. Wong;Sylvia C. Wong;Michael G. Johnson;Ji Ma;Robert Cho;L. Floren;K. Kersey;Karen Berry;Andrew P. Marcus;Xuemei Wang;Bettina van Lengerich;J. Medina;P. Pearson;B. Wong
通讯作者：
G. Tonn;Simon G. Wong;Sylvia C. Wong;Michael G. Johnson;Ji Ma;Robert Cho;L. Floren;K. Kersey;Karen Berry;Andrew P. Marcus;Xuemei Wang;Bettina van Lengerich;J. Medina;P. Pearson;B. Wong
影响因子：
4.9
作者：
Hueso, Luisa;Ortega, Rebeca;Piqueras, Laura
通讯作者：
Piqueras, Laura
影响因子：
8.6
作者：
Boks, Martine A.;Kager-Groenland, Judith R.;ten Brinke, Anja
通讯作者：
ten Brinke, Anja
影响因子：
2.5
作者：
Chen, Yonglin;Yin, Dekun;Xu, Zhongling
通讯作者：
Xu, Zhongling