MicroRNAs activate gene transcription epigenetically as an enhancer trigger.
MicroRNAs activate gene transcription epigenetically as an enhancer trigger.
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DOI：
10.1080/15476286.2015.1112487
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发表时间：
2017-10-03
期刊：
影响因子：
4.1
通讯作者：
Yu W
中科院分区：
文献类型：
--
作者：
Xiao M;Li J;Li W;Wang Y;Wu F;Xi Y;Zhang L;Ding C;Luo H;Li Y;Peng L;Zhao L;Peng S;Xiao Y;Dong S;Cao J;Yu W
microRNAs（miRNAs）是一类非编码的小分子RNA，具有负调控基因表达的功能。新的证据表明，除了在细胞质中发挥作用外，miRNA也存在于细胞核中。然而，核miRNAs的功能意义在很大程度上仍然不确定。通过对miRNA数据库的筛选，我们发现了一个具有增强子调节功能的miRNA亚类。在此，我们发现了一组具有基因激活功能的miRNAs。我们专注于miR-24-1，发现这种miRNA通过靶向增强子非常规地激活基因转录。因此，当TALEN删除增强子序列时，激活被完全消除。此外，我们发现miR-24-1激活增强子RNA（eRNA）表达，改变组蛋白修饰，并增加增强子位点p300和RNA Pol II的富集。我们的研究结果证明了miRNA作为增强子触发器的新机制。
MicroRNAs (miRNAs) are small non-coding RNAs that function as negative gene expression regulators. Emerging evidence shows that, except for function in the cytoplasm, miRNAs are also present in the nucleus. However, the functional significance of nuclear miRNAs remains largely undetermined. By screening miRNA database, we have identified a subset of miRNA that functions as enhancer regulators. Here, we found a set of miRNAs show gene-activation function. We focused on miR-24-1 and found that this miRNA unconventionally activates gene transcription by targeting enhancers. Consistently, the activation was completely abolished when the enhancer sequence was deleted by TALEN. Furthermore, we found that miR-24-1 activates enhancer RNA (eRNA) expression, alters histone modification, and increases the enrichment of p300 and RNA Pol II at the enhancer locus. Our results demonstrate a novel mechanism of miRNA as an enhancer trigger.
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影响因子：
64.8
作者：
Heintzman, Nathaniel D.;Hon, Gary C.;Hawkins, R. David;Kheradpour, Pouya;Stark, Alexander;Harp, Lindsey F.;Ye, Zhen;Lee, Leonard K.;Stuart, Rhona K.;Ching, Christina W.;Ching, Keith A.;Antosiewicz-Bourget, Jessica E.;Liu, Hui;Zhang, Xinmin;Green, Roland D.;Lobanenkov, Victor V.;Stewart, Ron;Thomson, James A.;Crawford, Gregory E.;Kellis, Manolis;Ren, Bing
通讯作者：
Ren, Bing
影响因子：
6.2
作者：
Majid, Shahana;Dar, Altaf A.;Saini, Sharanjot;Yamamura, Soichiro;Hirata, Hiroshi;Tanaka, Yuichiro;Deng, Guoren;Dahiya, Rajvir
通讯作者：
Dahiya, Rajvir
影响因子：
14.9
作者：
Huang V;Place RF;Portnoy V;Wang J;Qi Z;Jia Z;Yu A;Shuman M;Yu J;Li LC
通讯作者：
Li LC
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
7
作者：
Kelly TK;Liu Y;Lay FD;Liang G;Berman BP;Jones PA
通讯作者：
Jones PA