Increased bioactive lipids content in human subcutaneous and epicardial fat tissue correlates with insulin resistance.
Increased bioactive lipids content in human subcutaneous and epicardial fat tissue correlates with insulin resistance.
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DOI：
10.1007/s11745-012-3722-x
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发表时间：
2012-12
期刊：
影响因子：
1.9
通讯作者：
Gorski, Jan
中科院分区：
文献类型：
--
作者：
Blachnio-Zabielska, Agnieszka U.;Baranowski, Marcin;Hirnle, Tomasz;Zabielski, Piotr;Lewczuk, Anna;Dmitruk, Iwona;Gorski, Jan
肥胖是代谢性疾病的危险因素。神经酰胺、二酰基甘油和长链酰基辅酶A的肌内脂质积累是诱导胰岛素抵抗的原因。这些脂质可能不仅在骨骼肌而且在脂肪组织中与肥胖相关的胰岛素抵抗有关。关于人体皮下脂肪组织中神经酰胺含量的数据很少。然而，没有关于脂肪组织中DAG和LCACoA含量的数据。我们研究的目的是测量人SAT和心外膜脂肪组织中的脂质含量，我们试图通过LC/MS/MS确定来自瘦型非糖尿病、肥胖型非糖尿病和肥胖型糖尿病受试者的脂肪组织中的生物活性脂质含量，并测试脂质是否与HOMA-IR相关。我们发现，与LND组相比，OND和OD受试者两种类型脂肪组织中测量的脂质总含量显著更高（所有p < 0.001）。在SAT中，HOMA-IR与C16：0-Cer（r = 0.79，p < 0.001）和HOMA-IR与C16：0/18：2 DAG（r = 0.56，p < 0.001）之间存在正相关。在EAT中，我们发现C16：0-CoA含量与HOMA-IR之间存在强相关性（r = 0.73，p < 0.001）。研究表明，在肥胖和肥胖糖尿病患者中，皮下和心外膜脂肪组织中的生物活性脂质含量较高，并且特定脂质含量与HOMA-IR正相关。
Obesity is a risk factor for metabolic diseases. Intramuscular lipid accumulation of ceramides, diacylglycerols, and long chain acyl-CoA is responsible for the induction of insulin resistance. These lipids are probably implicated in obesity-associated insulin resistance not only in skeletal muscle but also in fat tissue. Only few data are available about ceramide content in human subcutaneous adipose tissue. However, there are no data on DAG and LCACoA content in adipose tissue. The aim of our study was to measure the lipids content in human SAT and epicardial adipose tissue we sought to determine the bioactive lipids content by LC/MS/MS in fat tissue from lean non-diabetic, obese non-diabetic, and obese diabetic subjects and test whether the lipids correlate with HOMA-IR. We found, that total content of measured lipids was markedly higher in OND and OD subjects in both types of fat tissue (for all p < 0.001) as compared to LND group. In SAT we found positive correlation between HOMA-IR and C16:0-Cer (r = 0.79, p < 0.001) and between HOMA-IR and C16:0/18:2 DAG (r = 0.56, p < 0.001). In EAT we found a strong correlation between C16:0-CoA content and HOMA-IR (r = 0.73, p < 0.001). The study showed that in obese and obese diabetic patients, bioactive lipids content is greater in subcutaneous and epicardial fat tissue and the particular lipids content positively correlates with HOMA-IR.
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一种液相色谱/串联质谱法，用于测量人体中血浆无脂肪酸的体内掺入人体中细胞内神经酰胺中。
影响因子：
2
作者：
Blachnio-Zabielska, Agnieszka U.;Persson, Xuan-Mai T.;Koutsari, Christina;Zabielski, Piotr;Jensen, Michael D.
通讯作者：
Jensen, Michael D.
影响因子：
6.9
作者：
Ahima, Rexford S.
通讯作者：
Ahima, Rexford S.
影响因子：
2.2
作者：
Blachnio-Zabielska, A.;Zabielski, P.;Gorski, J.
通讯作者：
Gorski, J.
影响因子：
5.6
作者：
Blachnio-Zabielska, Agnieszka;Baranowski, Marcin;Gorski, Jan
通讯作者：
Gorski, Jan
影响因子：
82.9
作者：
Erion DM;Shulman GI
通讯作者：
Shulman GI