Arabidopsis EMB1990 Encoding a Plastid-Targeted YlmG Protein Is Required for Chloroplast Biogenesis and Embryo Development.
Arabidopsis EMB1990 Encoding a Plastid-Targeted YlmG Protein Is Required for Chloroplast Biogenesis and Embryo Development.
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拟南芥 EMB1990 编码质体靶向 YlmG 蛋白是叶绿体生物发生和胚胎发育所必需的
DOI：
10.3389/fpls.2018.00181
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发表时间：
2018
影响因子：
5.6
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Chen H;Li S;Li L;Hu H;Zhao J
在高等植物中，胚胎发育起源于受精卵细胞，是生命周期的第一步。叶绿体参与许多重要的代谢途径，其功能与胚胎发育密切相关。然而，叶绿体在胚胎发生中的功能机制和相关的遗传成分在很大程度上是未知的。在本文中，我们描述了拟南芥EMB 1990基因，编码叶绿体生物发生和胚胎发育所需的质体靶向YlmG蛋白。EMB 1990/YLMG 1 -1缺失导致含有败育胚的白化病种子，并且纯合的emb 1990胚在球形期后的形态发育被破坏。结果表明，EMB 1990/YLMG 1 -1在胚状体发育过程中，主要表达于子叶原基和近轴区，其编码蛋白定位于叶绿体。透射电镜观察表明，emb 1990胚细胞叶绿体生物发生受到破坏。某些质体基因的表达也受到影响的功能丧失突变体，包括基因编码的核心蛋白复合物亚基位于类囊体膜。此外，胚胎发育的组织特异性基因在emb 1990突变体中错误表达，包括已知在SAM（茎顶端分生组织），子叶和垂体中描绘细胞命运决定的基因。综上所述，我们认为核编码的YLMG 1 -1是针对叶绿体的，并且是正常质体基因表达所必需的。因此，YLMG 1 -1通过参与叶绿体的生物发生在拟南芥胚胎发生中起着关键作用。
In higher plants, embryo development originated from fertilized egg cell is the first step of the life cycle. The chloroplast participates in many essential metabolic pathways, and its function is highly associated with embryo development. However, the mechanisms and relevant genetic components by which the chloroplast functions in embryogenesis are largely uncharacterized. In this paper, we describe the Arabidopsis EMB1990 gene, encoding a plastid-targeted YlmG protein which is required for chloroplast biogenesis and embryo development. Loss of the EMB1990/YLMG1-1 resulted in albino seeds containing abortive embryos, and the morphological development of homozygous emb1990 embryos was disrupted after the globular stage. Our results showed that EMB1990/YLMG1-1 was expressed in the primordia and adaxial region of cotyledon during embryogenesis, and the encoded protein was targeted to the chloroplast. TEM observation of cellular ultrastructure showed that chloroplast biogenesis was impaired in emb1990 embryo cells. Expression of certain plastid genes was also affected in the loss-of-function mutants, including genes encoding core protein complex subunits located in the thylakoid membrane. Moreover, the tissue-specific genes of embryo development were misexpressed in emb1990 mutant, including genes known to delineate cell fate decisions in the SAM (shoot apical meristem), cotyledon and hypophysis. Taken together, we propose that the nuclear-encoded YLMG1-1 is targeted to the chloroplast and required for normal plastid gene expression. Hence, YLMG1-1 plays a critical role in Arabidopsis embryogenesis through participating in chloroplast biogenesis.
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影响因子：
3.7
作者：
Lu X;Zhang D;Li S;Su Y;Liang Q;Meng H;Shen S;Fan Y;Liu C;Zhang C
通讯作者：
Zhang C
影响因子：
14.9
作者：
Heazlewood, Joshua L.;Verboom, Robert E.;Millar, A. Harvey
通讯作者：
Millar, A. Harvey
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影响因子：
6.4
作者：
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通讯作者：
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影响因子：
5.6
作者：
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通讯作者：
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影响因子：
4.5
作者：
Ke X;Zou W;Ren Y;Wang Z;Li J;Wu X;Zhao J
通讯作者：
Zhao J