Genetic Mapping by Integration of 55K SNP Array and KASP Markers Reveals Candidate Genes for Important Agronomic Traits in Hexaploid Wheat.
Genetic Mapping by Integration of 55K SNP Array and KASP Markers Reveals Candidate Genes for Important Agronomic Traits in Hexaploid Wheat.
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通过整合 55K SNP 阵列和 KASP 标记进行的遗传作图揭示了六倍体小麦重要农艺性状的候选基因
DOI：
10.3389/fpls.2021.628478
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发表时间：
2021
影响因子：
5.6
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Xiong H;Li Y;Guo H;Xie Y;Zhao L;Gu J;Zhao S;Ding Y;Liu L
抽穗期、株高、千粒重、穗长等农艺性状是影响小麦产量的重要因素。本研究利用Wheat55K SNP阵列构建了高密度遗传连锁图谱，对207个重组自交系的这些性状的数量性状位点（qtl）进行了定位。共鉴定出37个qtl，其中HD qtl 9个，PH qtl 7个，TGW qtl 12个，SL qtl 9个，解释了3.0-48.8%的表型变异。基于测序数据构建竞争等位基因特异性PCR （competitive Allele Specific PCR， KASP）标记，利用400个ril对3A、4B和6A染色体上稳定检测到的qtl进行验证。在4B染色体上，PH和TGW的QTL集群被划分为0.8 Mb的物理间隔，解释了12.2-22.8%的表型变异。基因注释和SNP效应分析表明，编码光合作用影响突变体68蛋白的基因是影响PH和TGW的候选基因，这是光系统II组装所必需的。此外，将3A染色体上HD的QTL缩小到2.5 Mb的间隔，推测一个编码R3H结构域蛋白的基因是影响HD的致病基因。本研究开发的连锁KASP标记可用于小麦育种中的标记辅助选择，候选基因为小麦这些性状的遗传研究提供了新的思路。
Agronomic traits such as heading date (HD), plant height (PH), thousand grain weight (TGW), and spike length (SL) are important factors affecting wheat yield. In this study, we constructed a high-density genetic linkage map using the Wheat55K SNP Array to map quantitative trait loci (QTLs) for these traits in 207 recombinant inbred lines (RILs). A total of 37 QTLs were identified, including 9 QTLs for HD, 7 QTLs for PH, 12 QTLs for TGW, and 9 QTLs for SL, which explained 3.0–48.8% of the phenotypic variation. Kompetitive Allele Specific PCR (KASP) markers were developed based on sequencing data and used for validation of the stably detected QTLs on chromosomes 3A, 4B and 6A using 400 RILs. A QTL cluster on chromosome 4B for PH and TGW was delimited to a 0.8 Mb physical interval explaining 12.2–22.8% of the phenotypic variation. Gene annotations and analyses of SNP effects suggested that a gene encoding protein Photosynthesis Affected Mutant 68, which is essential for photosystem II assembly, is a candidate gene affecting PH and TGW. In addition, the QTL for HD on chromosome 3A was narrowed down to a 2.5 Mb interval, and a gene encoding an R3H domain-containing protein was speculated to be the causal gene influencing HD. The linked KASP markers developed in this study will be useful for marker-assisted selection in wheat breeding, and the candidate genes provide new insight into genetic study for those traits in wheat.
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影响因子：
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作者：
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