Metabolomic approach for identifying and visualizing molecular tissue markers in tadpoles of Xenopus tropicalis by mass spectrometry imaging.
Metabolomic approach for identifying and visualizing molecular tissue markers in tadpoles of Xenopus tropicalis by mass spectrometry imaging.
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DOI：
10.1242/bio.019646
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发表时间：
2016-09-15
期刊：
影响因子：
2.4
通讯作者：
Mori T
中科院分区：
文献类型：
--
作者：
Goto-Inoue N;Kashiwagi A;Kashiwagi K;Mori T
在发育和细胞生物学中，评估代谢物的动态特征至关重要。一种使用热带爪蟾的新兴青蛙模型系统，其基因组序列和近交系已可用，现已准备好用于两栖动物的代谢组学研究。在这项研究中，我们应用基质辅助激光解吸/电离（MALDI）-质谱成像（MSI）分析来识别和可视化热带爪蟾蝌蚪中的代谢组分子标记。我们检测了组织特异性峰并可视化它们在组织中的分布，并区分了 19 种组织及其特定峰。我们首次通过串联质谱分析确定了它们的一些分子定位：动脉中的氢化可的松、菱脑中的左旋多巴、眼中的牛磺酸、鳃中的皮质酮、心脏中的血红素、肌肉中的肌苷单磷酸和肌肽、神经中的多巴胺以及咽中的磷脂酰乙醇胺（16:0/20:4）。这是首次针对热带蝌蚪的 MALDI-MSI 研究，因为在小蝌蚪中很难区分和解剖各种器官。此外，到目前为止还没有关于每个器官的代谢组学特征的数据。我们的结果表明，MALDI-MSI 可能是检查变态代谢组学动力学以及代谢变化引起的构象变化的强大工具。摘要：我们应用基质辅助激光解吸/电离质谱成像分析来识别和可视化热带爪蟾蝌蚪中的代谢组分子标记。我们在各种组织和细胞中发现了新的分子标记。
In developmental and cell biology it is crucial to evaluate the dynamic profiles of metabolites. An emerging frog model system using Xenopus tropicalis, whose genome sequence and inbred strains are available, is now ready for metabolomics investigation in amphibians. In this study we applied matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) analysis to identify and visualize metabolomic molecular markers in tadpoles of Xenopus tropicalis. We detected tissue-specific peaks and visualized their distribution in tissues, and distinguished 19 tissues and their specific peaks. We identified, for the first time, some of their molecular localizations via tandem mass spectrometric analysis: hydrocortisone in artery, L-DOPA in rhombencephalon, taurine in eye, corticosterone in gill, heme in heart, inosine monophosphate and carnosine in muscle, dopamine in nerves, and phosphatidylethanolamine (16:0/20:4) in pharynx. This is the first MALDI-MSI study of X. tropicalis tadpoles, as in small tadpoles it is hard to distinguish and dissect the various organs. Furthermore, until now there has been no data about the metabolomic profile of each organ. Our results suggest that MALDI-MSI is potentially a powerful tool for examining the dynamics of metabolomics in metamorphosis as well as conformational changes due to metabolic changes. Summary: We applied matrix-assisted laser desorption/ionization−mass spectrometry imaging analyses to identify and visualize metabolomic molecular markers in tadpoles of Xenopus tropicalis. We found new molecular markers in various tissues and cells.
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影响因子：
4.6
作者：
Goto-Inoue, Naoko;Yamada, Kenichiro;Inagaki, Akiko;Furuichi, Yasuro;Ogino, Shinya;Manabe, Yasuko;Setou, Mitsutoshi;Fujii, Nobuharu L.
通讯作者：
Fujii, Nobuharu L.
影响因子：
4.8
作者：
Kulkarni, Saurabh S.;Buchholz, Daniel R.
通讯作者：
Buchholz, Daniel R.
影响因子：
1.6
作者：
Goto-Inoue, Naoko;Setou, Mitsutoshi;Zaima, Nobuhiro
通讯作者：
Zaima, Nobuhiro
影响因子：
3.7
作者：
Vastag L;Jorgensen P;Peshkin L;Wei R;Rabinowitz JD;Kirschner MW
通讯作者：
Kirschner MW
影响因子：
8.8
作者：
Nishimura, Toshihide;Goto, Shingo;Wakabayashi, Hidehiko
通讯作者：
Wakabayashi, Hidehiko