Regulation of peptidoglycan synthesis by outer-membrane proteins.
Regulation of peptidoglycan synthesis by outer-membrane proteins.
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DOI：
10.1016/j.cell.2010.11.038
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发表时间：
2010-12-23
期刊：
影响因子：
64.5
通讯作者：
Vollmer W
中科院分区：
文献类型：
--
作者：
Typas A;Banzhaf M;van den Berg van Saparoea B;Verheul J;Biboy J;Nichols RJ;Zietek M;Beilharz K;Kannenberg K;von Rechenberg M;Breukink E;den Blaauwen T;Gross CA;Vollmer W
位于细菌内外膜（OM）之间的网状肽聚糖（PG）球囊的生长受到严格调控，以确保细胞完整性，维持细胞形状和协调分裂。细胞骨架元素直接从细胞内的PG脱氢酶的位置和活动，但对这一过程的精确时空控制知之甚少。我们证明PG合酶也受到球囊外部的控制。两种OM脂蛋白，LpoA和LpoB，分别对PBP 1A和PBP 1B的功能是必需的，主要的E. coli双功能PG脱氢酶。每种Lpo蛋白特异性结合其同源PBP并刺激其转肽酶活性，从而促进新PG与球囊的附着。LpoB显示部分隔膜定位和我们的数据表明，LpoB-PBP 1B复合物有助于OM收缩在细胞分裂过程中。LpoA/ LpoB及其PBP对接区域仅限于γ-变形菌，为球囊生长的生态位特异性调控提供了模型。
Growth of the meshlike peptidoglycan (PG) sacculus located between the bacterial inner and outer membranes (OM) is tightly regulated to ensure cellular integrity, maintain cell shape and orchestrate division. Cytoskeletal elements direct placement and activity of PG synthases from inside the cell but precise spatiotemporal control over this process is poorly understood. We demonstrate that PG synthases are also controlled from outside the sacculus. Two OM lipoproteins, LpoA and LpoB, are essential for the function respectively of PBP1A and PBP1B, the major E. coli bifunctional PG synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase activity, thereby facilitating attachment of new PG to the sacculus. LpoB shows partial septal localization and our data suggest that the LpoB-PBP1B complex contributes to OM constriction during cell division. LpoA/ LpoB and their PBP docking regions are restricted to γ-proteobacteria, providing models for niche-specific regulation of sacculus growth.
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影响因子：
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Cayley, DS;Guttman, HJ;Record, MT Jr
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Record, MT Jr
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von Mering C
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