Isoflurane induces Art2-Rsp5-dependent endocytosis of Bap2 in yeast.
Isoflurane induces Art2-Rsp5-dependent endocytosis of Bap2 in yeast.
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DOI：
10.1002/2211-5463.13302
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发表时间：
2021-11
期刊：
影响因子：
2.6
通讯作者：
Noda T
中科院分区：
文献类型：
--
作者：
Kozu F;Shirahama-Noda K;Araki Y;Kira S;Niwa H;Noda T
虽然全身麻醉在现代外科手术中是必不可少的，但吸入麻醉剂在分子和细胞水平上作用于突触膜的机制在很大程度上尚不清楚。在这项研究中，我们使用酵母细胞来检测异氟烷(一种吸入麻醉剂)对膜蛋白的影响。异氟烷处理酵母细胞时，BAP2是一种定位于细胞膜上的氨基酸转运蛋白。E3连接酶RSP5的耗尽阻止了这种内吞作用，Bap2在异氟醚的反应中被泛素化，表明这是一个泛素依赖的过程。对所有RSP5结合接头的筛选表明，Art2在这一过程中发挥着核心作用。这些结果表明，异氟烷通过Art2-Rsp5依赖的泛素化系统影响Bap2。BAP2是一种定位于酵母细胞质膜上的氨基酸转运蛋白，在异氟醚、全麻处理酵母细胞时，BAP2被内吞。E3连接酶RSP5和Art2(Arrestin)的缺失阻止了这种内吞作用和Bap2。因此，异氟烷通过依赖Art2-Rsp5的泛素化系统影响Bap2。
Although general anesthesia is indispensable during modern surgical procedures, the mechanism by which inhalation anesthetics act on the synaptic membrane at the molecular and cellular level is largely unknown. In this study, we used yeast cells to examine the effect of isoflurane, an inhalation anesthetic, on membrane proteins. Bap2, an amino acid transporter localized on the plasma membrane, was endocytosed when yeast cells were treated with isoflurane. Depletion of RSP5, an E3 ligase, prevented this endocytosis and Bap2 was ubiquitinated in response to isoflurane, indicating an ubiquitin‐dependent process. Screening all the Rsp5 binding adaptors showed that Art2 plays a central role in this process. These results suggest that isoflurane affects Bap2 via an Art2‐Rsp5‐dependent ubiquitination system. Bap2, an amino acid transporter localized on the plasma membrane in yeast, was endocytosed when yeast cells were treated with isoflurane, general anesthesia. Depletion of RSP5, an E3 ligase, and Art2 (arrestin) prevented this endocytosis and Bap2. Thus, isoflurane affects Bap2 via an Art2‐Rsp5‐dependent ubiquitination system.
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