Houttuynia cordata blocks HSV infection through inhibition of NF-κB activation.
Houttuynia cordata blocks HSV infection through inhibition of NF-κB activation.
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DOI：
10.1016/j.antiviral.2011.09.005
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发表时间：
2011-11
影响因子：
7.6
通讯作者：
Li E
中科院分区：
文献类型：
--
作者：
Chen X;Wang Z;Yang Z;Wang J;Xu Y;Tan RX;Li E
鱼腥草►houttuynia cordata Thunb.具有很强的抗单纯疱疹病毒活性。►H.Cordata通过抑制核因子-κB而不是丝裂原活化蛋白激酶来抑制单纯疱疹病毒2型感染。►中的主要活性成分为槲皮苷、槲皮苷和异槲皮苷等。鱼腥草Houttuynia cordata Thunb.是一种药用植物，在几个亚洲国家广泛用于民间医药。据报道，海参的水提物具有抗单纯疱疹病毒(HSV)和严重急性呼吸综合征(SARS)病毒的活性，但其作用机制尚不完全清楚。以往的研究表明，单纯疱疹病毒1型和2型的复制绝对需要核因子κB的激活，抑制核因子κB的激活可以抑制单纯疱疹病毒的感染。在这里，我们表明，热水提取物(HCWE)通过抑制核因子-κB的激活来抑制HSV-2感染。冻干粉的半数抑制浓度为50μg/ml。在150和450μg/ml浓度下，HCWE对感染性HSV-2的抑制作用分别大于3和4个对数。该抑制作用伴随着单纯疱疹病毒2型感染对NF-κB活性的抑制作用。尽管NF-κB和Erk MAPK的激活与单纯疱疹病毒的复制和生长有关，但HCWE对HSV-2诱导的Erk的激活没有影响。此外，我们还发现，由水提物中提取的主要黄酮类成分--栎皮苷、栎皮苷或异栎皮苷的处理能显著阻止HSV-2的感染。这些结果表明，海参通过抑制NF-κB的激活而抑制单纯疱疹病毒2型感染。
►Houttuynia cordata Thunb. exhibits strong activity against herpes simplex virus. ►H. cordata blocks HSV-2 infection through inhibition of NF-κB, not MAPK. ► Flavonoids like quercetin, quercitrin and isoquercitrin as major active components. Houttuynia cordata Thunb. is a medicinal plant widely used in folk medicine in several Asian countries. It has been reported that a water extract of H. cordata exhibits activity against herpes simplex virus (HSV) and the virus of severe acute respiratory syndrome (SARS), although the mechanisms are not fully understood yet. Previous studies have demonstrated absolute requirement of NF-κB activation for efficient replication of HSV-1 and HSV-2 and inhibition of NF-κB activation has been shown to suppress HSV infection. Here we show that a hot water extract of H. cordata (HCWE) inhibits HSV-2 infection through inhibition of NF-κB activation. The IC50 was estimated at 50 μg/ml of lyophilized HCWE powder. At 150 and 450 μg/ml, HCWE blocked infectious HSV-2 production by more than 3 and 4 logs, respectively. The inhibitory activity was concomitant with an inhibition of NF-κB activation by HSV-2 infection. Although activation of NF-κB and Erk MAPK has been implicated for HSV replication and growth, HCWE showed no effect on HSV-2-induced Erk activation. Furthermore, we show that treatment with quercetin, quercitrin or isoquercitrin, major water extractable flavonoids from H. cordata, significantly blocked HSV-2 infection. These results together demonstrated that H. cordata blocks HSV-2 infection through inhibition of NF-κB activation.
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影响因子：
2.7
作者：
HAYASHI, K;KAMIYA, M;HAYASHI, T
通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1248/yakushi1947.72.10_1227
发表时间：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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