Effect of bradykinin on TGF-β1-induced retinal pigment epithelial cell proliferation and extracellular matrix secretion.
Effect of bradykinin on TGF-β1-induced retinal pigment epithelial cell proliferation and extracellular matrix secretion.
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缓激肽对TGF-β1诱导的视网膜色素上皮细胞增殖和细胞外基质分泌的影响
DOI：
10.1186/s12886-016-0373-3
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发表时间：
2016-11-10
影响因子：
2
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Cai W;Wei Q;Liu Q;Ren C;Liu J;Zhang R;He M;Wang Q;Du Y;Yu J
To evaluate the effect of bradykinin (BK) on TGF-β1-induced retinal pigment epithelial (RPE) cell proliferation and extracellular matrix secretion and to elucidate the relationship between BK and the Erk/Akt signaling pathway. The effects of BK on TGF-β1-induced RPE cell proliferation were examined via CCK-8 assay. Cell culture supernatant collagen I concentrations were measured via ELISA. Fibronectin (Fn), matrix metalloproteinase-2 (MMP-2) and MMP-9 mRNA and protein expression levels were measured via q-PCR and Western blotting, respectively. Changes in Akt/Erk phosphorylation induced by BK and HOE-140 were evaluated via Western blotting. TGF-β1 stimulated ARPE-19 cell proliferation, which was inhibited by BK, whose effects were inhibited by HOE-140. BK inhibited TGF-β1-induced collagen I, Fn and MMP-2 secretion in RPE cells, and these effects were inhibited by HOE-140. BK also inhibited TGF-β1-induced Akt phosphorylation in RPE cells, and these effects were blocked by HOE-140. BK had no significant effect on Erk-mediated signaling. The findings from this study indicate that BK could be novel therapeutic targets for the treatment of PVR.
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影响因子：
3.7
作者：
Dvashi Z;Goldberg M;Adir O;Shapira M;Pollack A
通讯作者：
Pollack A
影响因子：
2.1
作者：
BAUDOUIN, C;FREDJREYGROBELLET, D;GASTAUD, P
通讯作者：
GASTAUD, P
DOI：
10.1152/ajprenal.00437.2011
发表时间：
2012-07-01
影响因子：
4.2
作者：
Blaes, Nelly;Pecher, Christiane;Girolami, Jean-Pierre
通讯作者：
Girolami, Jean-Pierre
影响因子：
3.7
作者：
Liu J;Feener EP
通讯作者：
Feener EP
影响因子：
0.4
作者：
Liu, C. Y.;Zhou, L. L.;Zhao, J. Y.
通讯作者：
Zhao, J. Y.