Histone methyltransferase ATX1 dynamically regulates fiber secondary cell wall biosynthesis in Arabidopsis inflorescence stem.
Histone methyltransferase ATX1 dynamically regulates fiber secondary cell wall biosynthesis in Arabidopsis inflorescence stem.
复制标题
组蛋白甲基转移酶 ATX1 动态调节拟南芥花序茎纤维次生细胞壁生物合成
DOI：
10.1093/nar/gkaa1191
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发表时间：
2021-01-11
影响因子：
14.9
通讯作者：
Luo K
中科院分区：
文献类型：
--
作者：
Wang X;Wang D;Xu W;Kong L;Ye X;Zhuang Q;Fan D;Luo K
在维管植物中，厚壁组织细胞的次生壁增厚受复杂的转录因子网络的严格控制。然而，调控次生壁生物合成的表观遗传机制尚不清楚。在本研究中，我们发现拟南芥同源的h3k4组蛋白甲基转移酶TRITHORAX1 （ATX1）介导了拟南芥花序茎纤维细胞壁发育的调控。全基因组分析显示，茎发育过程中参与二次壁形成的基因的上调在很大程度上是通过H3K4三甲基化水平的增加来协调的。在拟南芥H3K4me3的所有组蛋白甲基转移酶中，ATX1在花序茎发育过程中显著增加，功能缺失突变体ATX1在束间纤维的继发性壁增厚中受损。遗传分析表明，ATX1通过激活次级壁NAC主开关基因、次级壁相关NAC结构域蛋白1 （secondary wall - associated NAC DOMAIN PROTEIN1, SND1）和NAC次级壁增厚促进因子1 （secondary wall heening PROMOTING FACTOR1, NST1）的表达，积极调控次级壁沉积。我们进一步发现ATX1直接结合SND1和NST1的位点，并通过增加这些位点上的H3K4me3水平来激活它们的表达。综上所述，我们的研究结果表明，在拟南芥花序茎发育过程中，ATX1在束间纤维次生壁生物合成的调控中发挥了关键作用。
Abstract Secondary wall thickening in the sclerenchyma cells is strictly controlled by a complex network of transcription factors in vascular plants. However, little is known about the epigenetic mechanism regulating secondary wall biosynthesis. In this study, we identified that ARABIDOPSIS HOMOLOG of TRITHORAX1 (ATX1), a H3K4-histone methyltransferase, mediates the regulation of fiber cell wall development in inflorescence stems of Arabidopsis thaliana. Genome-wide analysis revealed that the up-regulation of genes involved in secondary wall formation during stem development is largely coordinated by increasing level of H3K4 tri-methylation. Among all histone methyltransferases for H3K4me3 in Arabidopsis, ATX1 is markedly increased during the inflorescence stem development and loss-of-function mutant atx1 was impaired in secondary wall thickening in interfascicular fibers. Genetic analysis showed that ATX1 positively regulates secondary wall deposition through activating the expression of secondary wall NAC master switch genes, SECONDARY WALL-ASSOCIATED NAC DOMAIN PROTEIN1 (SND1) and NAC SECONDARY WALL THICKENING PROMOTING FACTOR1 (NST1). We further identified that ATX1 directly binds the loci of SND1 and NST1, and activates their expression by increasing H3K4me3 levels at these loci. Taken together, our results reveal that ATX1 plays a key role in the regulation of secondary wall biosynthesis in interfascicular fibers during inflorescence stem development of Arabidopsis.
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影响因子：
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作者：
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通讯作者：
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