Effects of growth temperature upshift on cell cycle progression in Cryptococcus neoformans
Effects of growth temperature upshift on cell cycle progression in Cryptococcus neoformans
复制标题
生长温度升高对新型隐球菌细胞周期进程的影响
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
S. Kawamoto
中科院分区：
文献类型：
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作者：
K. Takeo;M. Ohkusu;S. Kawamoto
研究了在 15°、24° 和 30°C 下呈指数生长的细胞的新型隐球菌的细胞周期进程。除了速度之外，细胞周期进程相似。特别是，在 15°C、24°C 和 30°C 下 DNA 合成开始后相对较快就出现出芽。生长温度从 15°C 转变为 30°C 后，细胞在 DNA 合成开始前短暂停滞。因此，与酿酒酵母类似，“起始”是新生隐球菌中主要的易感细胞周期控制点。然而，在如上所述自发释放后，细胞进一步被捕获在未出芽状态。因此，出芽的时间被延迟到G2期之前，甚至直到进入G2期之后，但它也是短暂的，并且在DNA合成开始后的5小时后相对较快地出现芽。因此，新型隐球菌细胞可以通过延迟出芽来适应性地应对轻度应激。第二个敏感细胞周期控制点（即出芽）的存在似乎赋予了新型隐球菌一种独特的特征，即对增殖的抑制比对生长的抑制更强。还提出了新型隐球菌细胞周期的模型。
Cell cycle progression of Cryptococcus neoformans was studied for cells grown exponentially at 15°, 24°, and 30°C. Except for speed, cell cycle progression was similar. In particular, budding occurred relatively soon after initiation of DNA synthesis at 15°, 24°, and 30°C. After growth temperature was shifted from 15° to 30°C, cells were transiently arrested before initiation of DNA synthesis. Thus, similar to Saccharomyces erevisiae, “Start” was the main susceptible cell cycle controlling point in C. neoformans. However, after spontaneous release from arrest as above, cells were further arrested in the unbudded state. Thus, the timing of budding was delayed just before the G2 phase, or even until after entering the G2 phase, but it was also transient, and 5 h after the shift buds emerged relatively soon after initiation of DNA synthesis. Thus, C. neoformans cells can respond adaptively to mild stress by delaying budding. The existence of the second susceptible cell cycle control point, i.e., budding, appears to endow C. neoformans with a unique characteristic of stronger inhibition of multiplication than growth. A model of the C. neoformans cell cycle is also presented.
影响因子：
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作者：
L. Breeden
通讯作者：
L. Breeden
影响因子：
3.3
作者：
Ding, R;West, RR;McIntosh, JR
通讯作者：
McIntosh, JR