Rust secreted protein Ps87 is conserved in diverse fungal pathogens and contains a RXLR-like motif sufficient for translocation into plant cells.
Rust secreted protein Ps87 is conserved in diverse fungal pathogens and contains a RXLR-like motif sufficient for translocation into plant cells.
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铁锈分泌蛋白 Ps87 在多种真菌病原体中保守，并含有足以易位到植物细胞中的 RXLR 样基序
DOI：
10.1371/journal.pone.0027217
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Shan W
中科院分区：
文献类型：
--
作者：
Gu B;Kale SD;Wang Q;Wang D;Pan Q;Cao H;Meng Y;Kang Z;Tyler BM;Shan W
生物营养性植物病原真菌和卵菌的效应蛋白被传递到寄主细胞中，在疾病发生和抗病反应中发挥重要作用。专性真菌病原体效应物如何进入宿主细胞尚不清楚。条纹状锈菌的Ps87基因编码一种在多种真菌病原体中保守的蛋白质。据预测，含有锈菌的进化枝会分泌Ps87同源物。本研究的目的是测试Ps87是否可能在纹状锈菌感染中发挥效应。酵母信号序列诱捕试验表明，酵母细胞可以分泌锈病蛋白Ps87，而来自稻瘟病菌（Magnaporthe oryzae）的同源蛋白Ps87则无法分泌。细胞再入和蛋白摄取实验表明，Ps87含有保守的rxlr -样基序[K/R]RLTG的区域被证实能够将卵菌效应物Avr1b传递到大豆叶细胞，并将GFP传递到大豆根细胞。Ps87基序（KRLTG）的突变破坏了蛋白质的易位能力。结果表明，Ps87及其分泌的同源物可以利用与卵菌和其他真菌病原体相似的蛋白质转运机制。Ps87对植物的防御反应没有直接的抑制作用。这些结果表明Ps87可能代表了一种“新兴效应物”，它最近获得了进入植物细胞的能力，但尚未获得改变宿主生理的能力。
Effector proteins of biotrophic plant pathogenic fungi and oomycetes are delivered into host cells and play important roles in both disease development and disease resistance response. How obligate fungal pathogen effectors enter host cells is poorly understood. The Ps87 gene of Puccinia striiformis encodes a protein that is conserved in diverse fungal pathogens. Ps87 homologs from a clade containing rust fungi are predicted to be secreted. The aim of this study is to test whether Ps87 may act as an effector during Puccinia striiformis infection. Yeast signal sequence trap assay showed that the rust protein Ps87 could be secreted from yeast cells, but a homolog from Magnaporthe oryzae that was not predicted to be secreted, could not. Cell re-entry and protein uptake assays showed that a region of Ps87 containing a conserved RXLR-like motif [K/R]RLTG was confirmed to be capable of delivering oomycete effector Avr1b into soybean leaf cells and carrying GFP into soybean root cells. Mutations in the Ps87 motif (KRLTG) abolished the protein translocation ability. The results suggest that Ps87 and its secreted homologs could utilize similar protein translocation machinery as those of oomycete and other fungal pathogens. Ps87 did not show direct suppression activity on plant defense responses. These results suggest Ps87 may represent an “emerging effector” that has recently acquired the ability to enter plant cells but has not yet acquired the ability to alter host physiology.
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影响因子：
4.2
作者：
Tseng TT;Tyler BM;Setubal JC
通讯作者：
Setubal JC
DOI：
10.1126/science.1171652
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Panstruga R;Dodds PN
通讯作者：
Dodds PN
影响因子：
4.4
作者：
Godfrey, Dale;Bohlenius, Henrik;Thordal-Christensen, Hans
通讯作者：
Thordal-Christensen, Hans
影响因子：
9.2
作者：
Plett, Jonathan M.;Kemppainen, Minna;Martin, Francis
通讯作者：
Martin, Francis
影响因子：
11.6
作者：
Dou, Daolong;Kale, Shiv D.;Tyler, Brett M.
通讯作者：
Tyler, Brett M.